探討不同交聯程度與添加維他命E之超高分子量聚乙烯磨耗顆粒在小鼠顱骨對骨溶蝕之影響

人工關節因元件互相對磨所產生的超高分子量聚乙烯（UHMWPE）磨耗顆粒，在人體內會引發巨噬細胞吞噬，而釋放出發炎激素活化蝕骨細胞，導致骨溶蝕現象，最後造成人工關節的鬆脫。因此臨床上有使用交聯及交聯過後添加Vitamin E的UHMWPE，以改善UHMWPE的臨床現象。然而磨耗顆粒的大小、形貌、劑量皆會影響到誘發骨溶蝕的程度，因此本研究的目的為探討在大小、形貌相似及相同劑量下不同材料（UHMWPE、UHMWPE＋0.1％Vitamin E、70kGy HXLPE及70kGy HXLPE＋0.1％Vitamin E）磨耗顆粒對骨溶蝕的影響。本研究利用半導體製程中的微影與蝕刻，在四吋晶圓上製作微米加工表面的切割裝置，利用此裝置與不同的UHMWPE棒材進行加速磨耗測試，以量產出次微米尺寸之磨耗顆粒。利用這些磨耗顆粒在小鼠顱骨進行動物實驗，注射不同材料之次微米磨耗顆粒懸浮液，實驗兩週後以H&E stain、TRAP stain及3D micro-CT進行分析，結果顯示70kGy HXLPE有較多的骨溶蝕現象產生，但70kGy HXLPE＋0.1％Vitamin E的則有減少骨溶蝕的現象，因此交聯雖然能增加材料的抗磨耗性質，但有可能會引發較嚴重的骨溶蝕現象，但添加Vitamin E可能可以改善交聯過後所增加引發骨溶蝕的機會。

關鍵字

加工表面；超高分子量聚乙烯；交聯；抗氧化劑；骨溶蝕

並列摘要

UHMWPE wear particle-induced osteolysis has been recognized as the major cause of long-term failure in total joint replacement. These wear particles are phagocytosed by macrophages, which then release various inflammation cytokines stimulate activation of osteoclasts lead to prosthetic occur aseptic loosening. In order to decrease wear particles of UHMWPE are generated by wear .So enhance wear resistance and oxidization resistance of UHMWPE such as cross-linked and additional vitamin E. Therefore in this study, probe into the influence of high cross-linked and vitamin E containing UHMWPE wear particles on osteolysis in vivo. In this study,we applied microfabricated surface texture to rub against the pins of different UHMWPE in order to generate sub-micrometer wear particle.In vivo animal model,the skull of mice implement operation which injections of suspension of wear particle for two weeks after surgery implement analysis.Results from the animal study demonstrated the cross-linked UHMWPE induce more osteolysis than general UHMWPE.But the vitamin E containing UHMWPE can decrease cross-linked UHMWPE percentage of induce osteolysis.

並列關鍵字

surface texture ； UHMWPE ； cross-linked ； antioxidant ； osteolysis

參考文獻

37. 蘇毓智，生物潤滑液對超高分子量聚乙烯磨耗行為之影響，碩士論文，國立台北科技大學生物科技研究所，台北，2005。

3. J.A. D'Antonio, et al., "Five-year experience with Crossfire highly cross-linked polyethylene," Clinical Orthopaedics & Related Research, vol. 441, 2005, pp.143-150.

4. T. Asano, et al., "Dose Effect of Cross-Linking Polyethylene for Total Knee Arthroplasty on Wear Performance and Mechanical Properties," Journal of Biomedical Materials Research Part B: Applied Biomaterials, vol. 83B, no. 2, 2007, pp.615-622.

5. R.L. Illgen, et al., "Highly crosslinked vs conventional polyethylene particles: relative in vivo inflammatory response," J Arthroplasty, vol. 24, no. 1, 2009, pp.117-124.

6. R.L. Illgen, et al., "Highly crosslinked vs conventional polyethylene particles--an in vitro comparison of biologic activities," J Arthroplasty, vol. 23, no. 5, 2008, pp.721-731.

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探討不同交聯程度與添加維他命E之超高分子量聚乙烯磨耗顆粒在小鼠顱骨對骨溶蝕之影響

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