探討糖尿病鼠及高果糖餵食鼠之異常血液流變機轉

The mechanism of abnormal hemorheology in diabetic rat and high fructose-fed rat

陳銘宗(Ming-Tsung Chen)

指導教授 ： 方旭偉 ； 劉得任

國立臺北科技大學/工程學院/化學工程研究所/碩士(2008年)

摘要

本研究主要是以血液流變學(Hemortheology)模式評估糖尿病鼠及高果糖餵食鼠之各項血液流變參數，並討論造成其異常血液流變之機轉。
實驗分為糖尿病鼠及高果糖餵食鼠兩部份，均採用八周齡Wistar雄性老鼠。空腹十四小時之老鼠先經由腹腔注射nicotinamide(NA,180 mg/kg BW)以保護胰島素β細胞，十五分鐘後再腹腔注射streptozotocin(STZ,50 mg/kg BW)以誘發成糖尿病鼠。而高果糖餵食鼠則是直接給予21%高果糖水以誘發成X症候群，並以年齡匹配之正常鼠做為對照組別。實驗過程中之各項血液生化數值及血液流變參數均採空腹十四小時的血液進行檢測。
實驗結果顯示，糖尿病鼠及高果糖餵食鼠，從巨觀的全血黏度及MC-FAN到微觀的紅血球聚集度、紅血球變形度、全血自由基及血液生化數值皆異常於正常鼠。其中糖尿病鼠及餵食高果糖鼠之全血黏度、血漿中纖維蛋白原濃度、血清中總膽固醇、低密度脂蛋白膽固醇、高密度脂蛋白膽固醇、紅血球聚集度及全血自由基之值皆高於正常鼠，而紅血球變形度及MC-FAN所量測之體積流率則低於正常鼠。而引起糖尿病鼠與高果糖餵食鼠之血液於MC-FAN中所量測的體積流率變慢之主因為全血黏度的上升。而引起全血黏度的上升，則是因為紅血球於低剪切率下所表現出的高聚集度及紅血球於高剪切率下所表現出的低變形度。因此我們認為，可利用此兩種動物模式之異常血液流變機轉，建立其篩選藥物之動物血液流變平台

關鍵字

血液流變 ； 糖尿病 ； 餵食高果糖 ； 全血黏度 ； MC-FAN

並列摘要

The present study based on hemorheological model was to evaluate changes in hemorheological parameters of diabetes rats and normal rats fed with high fructose solution, and to discuss the possible mechanism response for these changes.
The animal study was divided into two sections: the diabetes rats and the high fructose-fed rats using 8-weeks-old male Wistar rats. The rats in the diabetes group was induced by a single intraperitoneal (i. p.) injection of 50 mg/kg streptozotocin, 15 min after the i.p administration of 180 mg/kg of nicotinamide which was used to protect the insulin-secreting beta-cells. The rats of the high fructose fed group were freely supplied with 21% fructose aqueous solution as a result of generating X syndrome. Rats at similar age were adopted as the controls. Blood samples were collected following 14 hours fasting and measured for biochemical parameters, complete blood counts and hemorheological parameters.
The results revealed that there were significant differences in all hemorheological parameters of the diabetes and the high fructose-fed groups from the control group. Whole blood viscosity, erythrocyte aggregation, fibrinogen levels, and free radicals in the diabetes and the high fructose-fed groups were significantly higher than that of the control group, while erythrocyte deformability and volume flow rate of MC-FAN of the diabetes and high fructose-fed groups were lower than that of the control group. The low volume flow rate measured in the diabetes and high fructose-fed groups was probably due to the rise of whole blood viscosity that was caused by high erythrocyte aggregation at low shear rate and low erythrocyte deformability at high shear rate. Therefore, we suggest that abnormal hemorheological properties generated by two animal models we employed in the present study could be developed as a useful animal hemorheological plateform for selection of drugs.

並列關鍵字

Diabetes ； Fructose-fed ； hemorheology ； whole blood viscosity ； MC-FAN

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