環狀RGD胜肽誘導人類乳癌細胞株MCF-7細胞死亡：Caspase路徑的影響

Cyclic RGD-Induced Cell Death in Human Breast Carcinoma (MCF-7) Cells: The Influence of Caspase Pathway

黃翠琴(Tsui-Chin Huang)

指導教授 ： 陳灝平 ； 阮雪芬

國立臺北科技大學/工程學院/生物科技研究所/碩士(2005年)

摘要

很多配位分子的intergrin辨識區域都發現是RGD motif，因此含RGD motif 的合成胜肽已經被廣泛當作integrin-ligand交互作用抑制劑，而用在細胞附著、遷移、生長和分化的研究上。我們設計了環狀RGD胜肽(Tpa-RGDWPC)，有較剛性的骨架，使其能與接受器更加緊密的結合。我們是利用固相合成法合成環狀RGD胜肽，而所用的固相支持物為Rink amide resin。我們先前的報告顯示環狀RGD胜肽會造成人類乳癌細胞株MCF-7死亡。在本研究我們利用DAPI染色法驗證環狀RGD會誘導人類乳癌細胞株MCF-7細胞凋亡。從cDNA微陣列的資料我們發現caspase 8 and FADD-like apoptosis regulator；caspase 9；細胞凋亡有關的蛋白質的抑制劑等等形成一個正向及負向的廻饋控制系統。這些結果也顯示了cRGD誘導細胞凋亡是經由caspase路徑。在本研究我們利用即時定量聚合酶連鎖反應(real-time quantitative PCR)的方法來觀察人類乳癌細胞株MCF-7處理cRGD不同時間後各個caspase mRNA的表現量，並且確認cRGD誘導細胞凋亡是經由caspase路徑且推估出caspase基因網路圖。

關鍵字

integrin ； 遷移 ； 細胞凋亡 ； caspase ； 即時定量聚合酶連鎖反應

並列摘要

Synthetic peptides containing the arginine-glycine- aspartate (RGD) motif have been used extensively as inhibitors of integrin-ligand interactions in studies of cell adhesion, migration,growth and differentiation, because the RGD motif is an integrin-recognition motif found in many ligands. we designed a cyclic-RGD peptide (Tpa-RGDWPC, cyclic-RGD) with rigid skeleton to bind closely to its acceptor. The cyclic-RGD was obtained by solid phase peptide synthesis method using Rink amide resin via a strategy .we previously reported that cyclic RGD caused cell death in MCF-7 cell line.Apoptosis was observed by 4', 6-diamidine-2`-phenylindole dihydrochloride (DAPI) staining for morphological changes for biochemical alterations. On the basis of cDNA microarray data, we suggest that the caspase 8 and FADD-like apoptosis regulator, the caspase 9 and inhibitor of apoptosis protein formed the positive and negative feedback control system. These results indicate that cyclic-RGD peptide can induce apoptosis by caspase pathway. The mRNA expression levels of all caspases in MCF-7 treated with cyclic RGD were analyzed and confirmed by real-time quantitative PCR technique.

並列關鍵字

integrin ； migration ； Apoptosis ； caspase ； real-time quantitative PCR

參考文獻

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