高壓靜電場對人類骨母細胞生長之影響

本篇論文探討人類骨母細胞株在利用交流靜電場，給予高電壓低電流的電刺激後產生的反應。以往曾有許多研究探討人類細胞在各種不同外界刺激下的反應，這些外加的刺激多半為直流靜電場，電磁場，直接通電（直流電或交流電），或是利用機械裝置所產生的應力，鮮少有研究觸及交流靜電場。本實驗利用MG-63細胞株作為研究對象，並將其置於1 kV，160 μA的交流靜電場中，利用多種測試方法，包括細胞活性測試，鹼性磷酸酶測試，Von Kossa染色法，亞硝酸鹽測試，反轉錄聚合酵素鏈鎖反應以及同步定量聚合酵素鏈鎖反應來評估交流靜電場對於人類骨母細胞生長之影響。研究的結果顯示在此條件之下，靜電場會抑制骨母細胞表現鹼性磷酸酶的能力，但對於第一型膠原蛋白以及骨鈣素基因之調控則呈現不同的結果。在此參數設定下之靜電場具有抑制第一型膠原蛋白基因活性，以及促進骨鈣素基因活性之能力。此外，細胞存活率和起始細胞密度有關，顯示初始細胞數量在人類骨母細胞生長過程中也是一個重要的因素。

關鍵字

交流靜電場；人類骨母細胞；同步定量聚合酵素鏈鎖反應

並列摘要

The aim of this study was to determine the effects of an AC electrostatic field generated from a device with high voltage and low current output on human osteoblastic cell line. Many studies about how human cells react and respond to external stimulations of various kinds and types were done before. Most of the external stimulations were DC electrostatic fields, electromagnetic fields, direct electrocution (DC or AC), or even mechanical loadings in these studies, but studies using AC electrostatic field as an external stimulation source were rarely found. This study utilized human osteoblasts as the target cells by putting them under the influence of an AC electrostatic field, which was set to use output parameters of 1 kV and 160 μA. The effects of electrostatic field on human osteoblastic cells were assessed by utilizing various kinds of tests, including cell viability test, alkaline phosphatase assay, Von Kossa staining, nitrite assay, reverse transcriptase PCR and real time quantitative PCR. The results showed that under current condition, the AC electrostatic field had a down-regulation effect on the production ability of alkaline phosphatase. Specific effects on different genes were discovered with the application of EFID. The expression level of osteocalcin gene was elevated while the expression level of type I collagen gene was reduced. In addition, cell viability showed a cell count-dependent result, suggesting initial cell density may also be a crucial factor in the proliferation process of human osteoblasts.

並列關鍵字

AC electrostatic field ； MG-63 ； real-time PCR

參考文獻

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高壓靜電場對人類骨母細胞生長之影響

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