In this work, the configuration and the output properties of a master oscillator power amplifier fiber laser are studied. In the experiment, the master oscillator, which is formed by a semiconductor laser associated with an elector-optic modulator, produces the seed pulses with pulse duration from 5ns to 80ns. The laser pulse is amplified by three-stage fiber amplifier to increase its peak power. Three kinds of gain fiber, including general erbium-doped fiber, high concentration erbium-doped and erbium-ytterbium co-doped large-mode-area double cladding fiber, are used in the three-stage fiber amplifier. For the single-stage and multi-stage fiber amplifiers, the effects of pump power and pulse repetition rate on the pulse properties, including pulse peak power and pulse duration, are discussed.