- 學位論文
利用奈米碳管感測器之生物晶片於結核桿菌檢測
Detection of mycobacterium tuberculosis by aptamer-based CNT biosensor
摘要
本研究主要為發展以單壁奈米碳管(single-wall-carbon nanotube, SWCNT)做為結核桿菌感測器，其製作方式是將3－aminopropyltriethoxysilane (APTES)修飾矽晶圓上之SiO2層，使其表面形成具有氨根aminoterminated (–NH2)的自我組裝單分子層(self-assembled monolayer, SAM)，接著浸泡於酸洗過之單壁奈米碳管溶液中，而經處理過的表面存在正電荷與氨基群能與CNTs產生化學的吸附作用，使CNTs固定於元件表面形成感測薄膜，接著利用微影製程與濺鍍的方式將梳狀電極沉積於其上，之後利用適體修飾碳管以完成CNTs結核桿菌感測元件的製作。 在本研究中利用適體對結核桿菌的專一性達到感測結核菌在微流體中感測的目的。當結核菌與適體結合時將產生微小電荷的傳遞，此時背閘極奈米碳管電晶體將有明顯的電流變化作為指標。將奈米碳管感測晶片與PDMS流道製程技術結合，在流道上做設計以degas封裝驅動毛細現象之流動，利用少量痰液與唾液可自行做肺結核檢驗並結合智慧型手機使得定點照護(point-of-care, POC)化的概念得以實現。結核桿菌(MTB)感測晶片，未來將製作成陣列形式，可同時偵測多種病原體之微流體自驅動藍芽讀取感測晶片。
並列摘要
Experiment substrate is a 4 inch wafer after APTS forms SAM and then it is immersed in NMP (N-methyl pyrrolidinone) solution with SWCNTs after pickling. Use lithography process and sputtering process to make the comb-shaped electrodes on the substrate. Modify SWCNTs with the sensing probes (Aptamers). EDC–NHS coupling chemistry was utilized for aptamers integration onto the SWCNTs. In this research, we used the aptamer specificity to detect the MTB in the droplets. Aptamer is an synthetic oligonucleotide which can combine with different substance (such as metal ion, molecule, protein.etc.), the selectivity, specificity and the affinity of it is the same as the other anti-body, even better than them. As the MTB combined with the aptamer, there will be a significant change in the current of the CNTFET(Carbon-Nano-Tubes Field Effect Transistor). We had used the PDMS process to produce a bio-sensor chip. We expect that the bio-sensor could make in array form, and had the ability that can detect different bacterium at the same time in the future.