膠原蛋白/四鈣磷酸鹽複合骨膠之研究

本研究旨在開發一新式複合骨膠，其以四鈣磷酸鹽 (TTCP)並加入第一型膠原蛋白及羧甲基纖維素製成骨膠，於初期具有塑性，後期可水合硬化提供強度。四鈣磷酸鹽有快速水合的能力使其在短時間內硬化，具有良好的骨質結合能力。第一型膠原蛋白，主要分布於皮膚、硬骨及血管等多數結締組織，在組織重建、傷口癒合便演種要角色。羧甲基纖維素是纖維素基環上氫氧基被甲基取代之衍生物。根據不同的取代度產生不同程度的黏稠性質，近年來廣泛被應用於生醫產品中。本實驗使用CPC混合膠原蛋白5~40% ( Col 5~Col 40 )，做基本物理性質、生物相容性測試及藥物制放實驗，結果顯示Col 5具有良好之流動性質及剪切黏附力，有成為注射性骨膠的潛力，而Col 40由於膠原蛋白含量較高，造成材料吸水膨脹進而影響材料之貼附性質，細胞毒性測試呈現材料無毒之。藥物制放顯示材料在浸泡24小時內，藥物制放率快速上升，而在浸泡2天後，藥物釋放率呈現穩定狀態。由此可見，材料適合作為萬古黴素之載體。抑菌圈顯示經由材料所釋出之四環黴素仍有抑菌效果。綜合以上結果，Col 5 具有足夠機械強度及貼附力，且無細胞毒性，在藥物制放實驗中顯示材料適合作為抗生素之載體，具有成為骨膠複合材料之潛力。

關鍵字

骨膠；四鈣磷酸鹽；二鈣磷酸鹽；第一型膠原蛋白；羧甲基纖維素；注射型骨膠

並列摘要

The purpose of this study is to develop new composite glue, mainly composed of tetracalcium phosphate (TTCP) and type I collagen, which is deformable in the early stage and becomes strengthened after hydration. TTCP is able to rapidly self-set and harden after hydration. The product is osteoconductive. Type I collagen, the majority distribution of connective tissue in the skin, hard bone and blood vessels etc. , plays an important role in tissue remodeling and wound healing. Carboxymethyl cellulose (CMC) is a cellulose derivative with carboxymethyl groups (-CH2-COOH) bound to some of the hydroxyl groups of the glucopyranose monomers that make up the cellulose backbone. Varied degree of substitution provides unique viscosity, so that CMC is applied as thermo-responsive hydrogel in the recent studies. Sodium carboxymethyl cellulose with medium degree of substitution was used to increase adhesion ability of the bone glue in this research. A series characteristics were carried out, including mechanical properties test, surface morphology observation, crystalline phase analysis, pH value variation and in vitro test.

並列關鍵字

Bone glue ； Tricalcium silicate ； Type I Collagen ； Carboxymethyl cellulose ； Bone graft

參考文獻

[1] 莊玲菁，「治療骨質疏鬆症的新藥物」，彰基藥訊，第27期-89，八卷，四期，2000。

[3] Fujibayashi S., Kim HM., Neo M., Uchida M., Kokubo T., Nakamura T. Repair of segmental long bone defect in rabbit femur using bioactive titanium cylindrical mesh cage. Biomaterials, 24, 2003, 3445-3451.

[4] Imranul Alam, M., Asahina, I., Ohmamiuda, K., Takahashi, K., Yokota, S., Enomoto, S.. Evaluation of ceramics composed of different hydroxyapatite to tricalcium phosphate ratios as carriers for rhBMP-2. Biomaterials, 22, 2001, 1643-1651.

[5] Kikuchi, M., Itoh, S., Ichinose, S., Shinomiya, K., Tanaka, J., Self-organization mechanism in a bone-like hydroxyapatite/collagen nanocomposite synthesized in vitro and its biological reaction in vivo. Biomaterials, 22, 2001, 1705-1711.

[6] Saito, N., Takaoka, K. New synthetic biodegradable polymers as BMP carriers for bone tissue engineering. Biomaterials, 24, 2003, 2287-2293.

國際替代計量

膠原蛋白/四鈣磷酸鹽複合骨膠之研究

全文下載

主題瀏覽