光磁性核殼結構奈米顆粒之製備方法及與DNA結合之研究

本研究旨在發展具有光磁性質的奈米核殼結構，並將其與不同序列之DNA 作雜合反應，以便應用於標靶藥物治療及生醫相關領域等方面。先以熱迴流法製備出平均粒徑為5 nm以下之鐵鉑磁性奈米流體，由於鐵鉑奈米粒子的表面呈現帶負電的狀態，所以我們利用正負電相吸的原理，讓表面帶有正電的硫化鋅吸附於鐵鉑奈米顆粒上面，以形成核殼結構。硫化鋅本身具有螢光性質，可以提升鐵鉑奈米粒子的光學性；加上鐵鉑顆粒本身具有磁性，而且呈現超順磁的狀態，所以我們可以製備出具有光學和磁性的複合結構核殼奈米粒子。接著我們將兩組不同序列的DNA與製備而成的核殼結構奈米粒子做結合，因為DNA本身帶有微量負電，所以我們同樣以吸附原理將DNA吸附於奈米粒子上面；最後再用一組橋接DNA跟兩組不同序列的DNA做雜合反應，以此將其連接在一起。最後我們用TEM和XRD等儀器量測核殼奈米粒子的大小和成分；以SQUID量測其磁滯曲線；用UV-vis分光光度計證明流體內確實有DNA的存在；然後利用螢光光譜儀的檢量線算出雜合的效率。除此之外，我們還將探討添加轉相劑和FePt奈米流體的多寡對製程的影響。

關鍵字

FePt磁性奈米粒子； ZnS ；核殼結構； DNA ；雜合

並列摘要

This study aims to develop a nanocore-shell structure with photomagnetic nature and make it conjugate with the DNAs of different sequences to apply the structure to target-based drug therapy and biomedical-related areas. First, iron platinum (FePt) magnetic nanofluid with average size below 5 nm is prepared by the hot reflux method. Because the surface of FePt nanoparticles is negative, the principle of positive and negative charges attraction is used to make zinc sulfide (ZnS) with positive surface attach to FePt nanoparticles to form the core-shell structure. ZnS can elevate optical properties of FePt nanoparticles due to its fluorescent characteristics. FePt particles have magnetism and display superparamagnetic situation and thus, compound nanoparticles with core-shell structure with optical and magnetic properties cab be fabricated. Two different sequences of DNAs and prepared nanoparticles with core-shell structure are conjugated. With slight negative electricity by DNA itself, DNA attaches to nanoparticles according to adsorption theory and then, a bridge DNA and two sequences of DNAs hybridize to connect. Finally, transmission electron microscopy (TEM) and X-ray diffraction (XRD) are adopted to measure the size and ingredients of nanoparticles with core-shell structure. Superconducting quantum interference device (SQUID) is used to measure the hysteresis curve and UV-vis spectrophotometer is employed to prove that DNA exists in fluid. Fluorescence spectrometer is used to calculate the efficiency of hybridization. Besides, we also deal with the influence of adding phase transfer agent and FePt nanofluid on manufacturing process.

並列關鍵字

FePt magnetic nanoparticles ； ZnS ； Core-shell structure ； DNA ； Hybridization

參考文獻

[4] G. A. Ozin, Adv. Mater., vol.4, 1992, pp.612-649.

[7] A. Arakaki, S. Hideshima, T. Nakagawa, D. Niwa, T. Tanaka, T. Matsunaga, T. Osaka, “Detection of Biomolecular Interaction Between Biotin and Streptavidin on a Self-Assembled Monolayer Using Magnetic Nanoparticles”, Biotechnol. Bioeng. 88, 2004, pp.543-546.

[8] B. G. Nidumolu, M. C. Urbina, J. Hormes, C. S. S. R. Kumar, W. T. Monroe, “Functionlization of Gold and Glass Surface with Magnetic Nanoparticles Using Biomolecular Interctions ”, Biotechnol. Bioeng, vol.22, 2006, pp.91-95.

[9] H. P. Khng, D. Cunliffe, S. Davies, N. A.Turner, E. N.Vulfson, “The synthesis of sub-micron magnetic particles and their use for preparative purification of proteins,”Biotechnol. Bioeng. 60, 1998, pp.419-424.

[10] B. Szewczyk, Z. Pilat, K. Bienkowska-Szewczyk, D. F. Summers, “Elution of glycoproteins from replicas of sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels,” Electrophoresis, vol.19, 1998, pp.220-223.

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光磁性核殼結構奈米顆粒之製備方法及與DNA結合之研究

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