石墨烯型電化學感測器與生物感測器之設置與定性

Fabrication and Characterization of Graphene Based Eletrochemical Sensors and Biosensors

binesh unnikrishnan(Binesh Unnikrishnan)

指導教授 ： 陳生明

國立臺北科技大學/工程學院/工程科技研究所/博士(2012年)

https://doi.org/10.6841/NTUT.2012.00307

摘要

此篇論文探討了石墨烯及奈米材料修飾電極的製備及特性，可用以偵測各種重要的生物成分，例如葡萄糖、過氧化氫、Carbamazepine、對苯二酚、兒茶酚等等。氧化石墨烯的基面及邊緣有許多含氧的官能基，可讓酵素有效的固定其上。Graphene-glucose oxidase生物複合物及horseradish peroxidase生物複合物可使用簡單新穎的電化學方法合成，可用以發展葡萄糖及過氧化氫生物感測器。此感測器製作簡單、選擇性高、線性範圍好。首先graphene-enzyme生物複合薄膜合成於玻璃碳電極上。再將其放在pH5磷酸緩衝溶液中，使用電化學還原法將複合物內的氧化石墨烯還原為還原氧化石墨烯。石墨烯-單層奈米碳管(Graphene-SWCNT)複合物使電化學還原製備。此電化學感測器可用來偵測抗癲癇藥物Carbamazepine。Horse radish peroxidase multimer與 Carbodiimide耦合反應修飾於電極上，包含N-ethyl-Nʹ-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS)。HRP multimer 修飾電極對雙氧水有很好的電催化活性，偵測真實樣本也有很好的表現。

關鍵字

安培生物感測器 ； 酵素 ； 直接電化學 ； 奈米材料 ； 電催化

並列摘要

This thesis study report the fabrication and characterization of modified electrodes based on graphene, graphene oxide, reduced graphene oxide and single walled carbon nanotubes for the determination of various biologically important compounds such as glucose, hydrogen peroxide and carbamazepine. Graphene oxide has plenty of oxygen containing functional groups both at its basal plane and edges. The plenty of oxygen containing functional groups on graphene have been used for the effective immobilization of the enzymes. Graphene-glucose oxidase biocomposite and graphene-horse radish peroxidase biocomposite have been synthesized by a simple and novel electrochemical approach for the development of amperometric glucose and hydrogen peroxide biosensors. The sensors have the advantages of ease of fabrication, good selectivity and linear range of detection. In this method of fabrication, graphene-enzyme biocomposite has been synthesized and a thin film was prepared out of it on glassy carbon electrode and screen printed carbon electrode. Then the graphene oxide in the composite has been reduced to reduced-graphene oxide by electrochemical reduction in pH 5 phosphate buffer solution. Graphene-single walled carbon nanotube composite has been prepared by a simple solution method followed by electrochemical reduction. An efficient amperometric sensor has been developed for the determination of the epileptic drug Carbamazepine. Horse radish peroxidase multimer has been fabricated on graphene modified electrode with carbodiimide coupling reaction involving N-ethyl-Nʹ-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The HRP multimer modified electrode shows good electrocatalytic activity towards H2O2. It shows promising performance in real sample analysis.

並列關鍵字

Amperometric bio-sensors ； Enzyme ； Direct electrochemistry ； Nano Materials ； Electrocatalysis

參考文獻

[4] J. Wang, Electroanal., 17 (2005) 7.

[6] W. Tischer and F. Wedekind, Immobilized enzymes: Methods and applications, Biocatalysis from Discovery to Application Topics in Current Chemistry, 200 (1999) 96.

[8] T. Godjevargova, R. Nenkova and N. Dimova, Macromol. Biosci., 5 (2005) 760–766.

[11] L. S. Wan, B. B. Ke and Z. K. Xu, Enzyme Microb., Technol. 42 (2008) 332.

[12] R. A. Sheldon, Adv. Synth. Catal., 349 (2007) 1289.