以電紡與冷凍乾燥技術製備雙層膠原蛋白軟骨支架並比較非順向與順向之奈米纖維

Fabrication of bi-layer collagen cartilage scaffolds by electrospinning and freeze-drying and comparison of the difference between the random and the aligned nano-fibers

蔡文啟(Wen-Chi Tsai)

指導教授 ： 林忻怡

國立臺北科技大學/工程學院/化學工程研究所/碩士(2013年)

摘要

軟骨組織工程是一種應用於受損軟骨組織之組織修復及再生的新技術。近年來利用組織工程在體外培養軟骨組織已成為修補受損關節軟骨的途徑之一，基本概念是將細胞取出，並於合適的基材及環境下進行體外培養，再植入體內，透過此方式，獲得近似於天然的軟骨組織，修補軟骨受損部位。
　　軟骨是由僅佔總體積百分之五的軟骨細胞和所分泌的胞外基質所組成，大約分成四層，每層的細胞型態、分佈及密度皆略有差異。其細胞外基質主要成份為第二型膠原蛋白和蛋白醣。軟骨組織的自我修復以及再生能力極差，一旦產生創傷，軟骨細胞不易有效地至受傷部位進行修復。故利用組織工程技術有效地修復受損軟骨組織為本實驗所期待的目標。
　　本實驗以膠原蛋白為基材，利用冷凍乾燥及靜電紡絲技術製作仿軟骨結構之多孔性支架，再經戊二醛進行交聯處理，提高支架機械性質。並進行物理性質測試及體外細胞培養來探討本實驗支架對於軟骨組織修復之影響。實驗結果顯示，本實驗支架具有穩定的結構及機械強度，於體外細胞培養結果得知，本實驗支架能有效使細胞貼附並促進細胞增生，具良好的生物相容性。

關鍵字

膠原蛋白 ； 靜電紡絲 ； 冷凍乾燥 ； 軟骨細胞

並列摘要

Tissue engineering of cartilage is emerging as a technique for the regeneration or repair of damaged cartilage tissue. Tissue engineering has been a feasible way to regenerate cartilage in vitro. The fundamental concept of tissue engineering includes cell isolating, cell culturing in vitro, and implanting in vivo. To obtain nearly native articular cartilage tissue and repair the damaged section are currently the main purposes of cartilage tissue engineering.
　　Articular cartilage tissue is composed of chondrocytes and extracellular matrix, where the chondrocytes only make up about 5% of the total volume of cartilage. Articular cartilage is comprised of four different layers that can be distinguished from one another by the morphology, distribution and density of chondrocytes. The matrix is composed of collagens, especially type II collagen, and proteoglycan. The proliferation and self-repair of articular cartilage are both poor, because chondrocytes can not migrate to the damaged tissue to repair effectively when injured. So, repair the damaged cartilage tissue effectively by the techniques of tissue engineering is the purpose of this study.
　　In this study, we fabricate the porous scaffolds base on collagen for imitating native cartilage tissue by freeze-drying and electrospinning, and cross-link with glutaraldehyde to raise the mechanical properties. And explore the influence of repair of cartilage tissue by physical properties tests and in vitro cell culturing tests. The results indicate that the scaffolds have stable structure and mechanical strength, and in vitro cell culturing tests showed that the scaffolds are able to effectively promote cells attachment and proliferation, and with good biocompatibility.

並列關鍵字

collagen ； electrospinning ； freeze-drying ； chondrocyte

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