- 學位論文
以離子液體修飾鎳電極製作生物感測器及Sn-Sb-Zn 三元系統相平衡之研究
A Study of a Biosensor Made of Nickel Electrode Immobilized by Ionic Liquid and the Phase Equilibrium of Sn-Sb-Zn Alloy
摘要
本研究分為兩部分，第一部分為生物感測器的製作。製程包括用網印及濺鍍製備三極式平面電極，然後用滴覆的方式，分別滴上奈米碳管溶液、幾丁聚醣-離子液體溶液與酵素溶液，成功製備出葡萄糖感測器。利用傅立葉紅外線光譜儀確認奈米碳管與離子液體可以穩定地固定在電極表面。接著使用循環伏安法來對電極作分析，藉由改變掃描速率，得知此電化學系統的控制機制為擴散。對不同修飾層做循環伏安法的分析後，得知奈米碳管能有效增加電子傳遞，幾丁聚醣與幾丁聚醣-離子液體薄膜則會阻礙電子傳遞。相對比較之後，得知離子液體是可以增加葡萄糖偵測的電流響應。本研究所製備出葡萄糖感測器，其線性範圍為2 ∼ 10 mM，靈敏度為9.87
並列摘要
This thesis contained two studies and the first study was to make a biosensor for testing the concentration of glucose. The sensor adopted nickel as one of the electrodes and used an ionic liquid to immobilize an enzyme. In this study, glucose oxidase was used as enzyme. Since chitosan and ionic liquid would decrease the response current, carbon nanotube was applied on the electrode surface to enlarge the conducting path and therefore increased the response current. The control mechanism of the electrochemical system of this biosensor was proved to be a diffusion process. A linear range could therefore be obained in this sensor. The characteristic properties of this biosensor were as follows：Linear range = 2∼10 mM, Sensitivity = 9.87