以快速原型技術製作3D多孔性幾丁聚醣/膠原蛋白支架應用於皮膚組織工程

Fabrication 3D Porous Chitosan/Collagen Scaffold by Rapid Prototyping Technique for Applications in Skin Tissue Engineering

張雅瑄(Ya-Hsuan Chang)

指導教授 ： 林忻怡

國立臺北科技大學/工程學院/生化與生醫工程研究所/碩士(2013年)

摘要

組織工程是藉由多孔、可被降解的支架運送細胞、基因、蛋白質，使受傷的組織達到組織修復的目的。組織工程中，支架用來作為胞外基質的替代物，引導細胞向內生長並使組織再生。
理想的支架需具有適當的表面化學特性，使細胞貼附、增生及分化；大量互通的孔洞所形成的網狀結構，可使氧氣和營養物質通過提供細胞生長；足夠的機械強度及生物降解率；高度生物相容性可使細胞貼附及生長。
傳統的支架製作方法不能精確的控制孔洞大小、形狀、互通性及孔洞的在立體空間分布。利用快速原型(Rapid prototpying)技術製作的支架具有互相通聯的孔洞，有利於營養物質的輸送、代謝物的排除；高度多孔的特性，則利於細胞生長與組織生成。
本研究以快速原型技術製作幾丁聚醣/膠原蛋白支架作為實驗組、幾丁聚醣支架為對照組，比較兩種支架在物理性質及生物相容性上的差異性。
實驗結果顯示，以快速原型技術製作的幾丁聚醣/膠原蛋白支架結構具有穩定的機械強度，支架的多孔性使其具良好的生物降解性及生物相容性，並可以促進纖維母細胞生長達到皮膚修復的功能。

關鍵字

快速原型 ； 幾丁聚醣 ； 膠原蛋白 ； 纖維母細胞

並列摘要

Tissue engineering that repairs damage tissues through the porous, degradable scaffold to deliver cells, genes, and proteins. In tissue engineering, scaffolds serve as temporary surrogates for the extracellular matrix and guide for tissue ingrowth to regenerate the tissue.
The ideal scaffolds should have (Ⅰ) An appropriate surface chemistry characteristics to facilitate cell attachment, proliferation and differentiation. (Ⅱ) An extensive network of interconnecting pores through which oxygen and nutrients are provided cells to grow. (Ⅲ) Adequate mechanical strength and biodegradation rate. (Ⅳ) High biocompatibility for cells to attach and proliferate.
Conventional techniques methods do not control precisely over the pore size, pore geometry, pore interconnectivity and the spatial distribution of pores, Using rapid prototyping technology to facbricate scaffold which has interconnected porous and facilitate transport of nutrients and metabolite into and out of the scaffold. Highly porous of scaffolds facilitate cell growth and tissue formation.
In the present study, we fabricated chitosan/collagen scaffold as the exprientment group and chitosan scaffold as the control group. Further, we compared two scaffolds differences between physical characteristics and biocompatibility characteristics.
Results show that using rapid prototyping technology to fabricate the chitosan /collagen scaffold which structure has stable mechanical strength, and which porous properties has good biodegradability and biocompatibility which can promote fiberblast growth to repair skin.

並列關鍵字

rapid prototyping ； chitosan ； collagen ； fibroblast

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