奈米顆粒結合生物型交聯結構與去氧核醣寡核甘酸之雜合檢測

本文在發展一種具磁特性之交聯結構，結合生物相容性之結構而合成出奈米複合顆粒。本研究所發展之交聯結構，其磁性可應用於生醫相關領域，也可以用於特定組織之偵測細胞及輔助顯影功用或應用於標靶藥物治療等方面。以化學沉積法所製備出之純淨Fe3O4奈米磁性流體，其平均粒徑約為18 nm，並且於Fe3O4顆粒表面上吸附一層生物型複合結構幾丁聚醣/藻酸鈉(CS/ALG)交聯物。由顆粒表面電位之檢測結果顯示，Fe3O4顆粒表面吸附一層生物型交聯物後帶有正電，因此可增加與去氧核糖寡核甘酸結合之效率。本研究將製備出之交聯奈米複合顆粒與兩組不同序列之去氧核糖寡核甘酸及ㄧ组橋去氧核糖寡核甘酸連結，並混合於培養皿中，這三種不同序列之寡核苷酸片段鹼基(base)係設計成部分互補，藉由ODN之間的雜合反應而使之互相連接在一起；並且於反應後分離出未鍵結於Fe3O4@ CS/ALG 交聯顆粒表面之去氧核糖寡核甘酸片段，以及未發生雜合反應之去氧核糖寡核甘酸，而僅保留已互相連接及產生雜合反應之產物，經由各種檢測儀器證實，Fe3O4顆粒係相互緊靠在一起，並進一步分析此交聯結構之各項特性；另以Raman光譜儀分析經雜合反應後的流體中，確定有ODNs存在；同時計算出不同濃度ODNs之雜合效率。

關鍵字

Fe3O4奈米磁性流體；幾丁聚醣/藻酸鈉；交聯結構；去氧核糖寡核甘酸；雜合效率

並列摘要

This study aims to develop a kind of crosslinked structure with magnetism and combine with a biocompatible structure to synthesize compound nanoparticles. The crosslinked structure developed from this study whose magnetism can be applied in biomedical field, in detection cells of specific tissues, assisted imaging or targeted drug therapy. Pure Fe3O4 magnetic nanofluid is fabricated by chemical deposition method, with average size of 18 nm and covered by a layer of CS/ALG shell with a biotype compound structure. Results of the detection of surface potential of particles show that the surface of Fe3O4 particles is covered by a layer of biotype conjugates with positive electricity to increase the conjugation efficiency of ODNs. This study connects compound nanoparticles with crosslinked structure with two groups of different sequences of ODNs and a bridge of ODN and mixes them in a petri dish. The bases of three different sequences of ODN fragments complement one another and link by hybridization of ODNs. After reaction, ODN fragment are separated from without being covered on the surface of Fe3O4@ CS/ALG crosslinked particles and ODNs without being hybridized. Only of linked and hybridized products are reserved. Results show that Fe3O4 particles are close with each other and the properties of crosslinked structure are further analyzed. Besides, fluid after hybridization measured by Raman spectroscopy proves the existence of ODNs. Simultaneously, hybridization efficiency of ODNs with different concentration is calculated as well.

並列關鍵字

Fe3O4 nanoferrofluid ； CS/ALG ； Crosslinked ； ODNs ； Hybridization efficiency

參考文獻

[1] 陳播暉，幾丁聚醣/果膠複合材料之製備與特性探討，碩士論文，國立台灣大學化學工程所，臺北市羅斯福路四段一號，(2004)。

[2] Ningning Zhu Hong Cai Pingang He Yuzhi Fang “Tris(2,2_-bipyridyl)cobalt(III)-doped silica nanoparticle DNA probe for the electrochemical detection of DNA hybridization” Analytica Chimica Acta, vol.481, 2003, pp. 181–189.

[3] Wu Lu, Yan Jin, Geng Wang, Da Chen, Jinghong Li “Enhanced photoelectrochemical method for linear DNA hybridization detection using Au-nanopaticle labeled DNA as probe onto titanium dioxide electrode” Biosensors and Bioelectronics, vol.23,2008, pp. 1534–1539.

[4] V. Lavalley, P. Chaudoue¨t, V. Stambouli “An atomic force microscopy study of DNA hairpin probes monolabelled with gold nanoparticle: Grafting and hybridization on oxide thin films” Surface Science ,vol.601,2007, pp.5424–5432.

amplified by nanoparticles” Biosensors and Bioelectronics,vol.21,2006, pp.1727–1736.

國際替代計量

奈米顆粒結合生物型交聯結構與去氧核醣寡核甘酸之雜合檢測

未授權

主題瀏覽