- 學位論文
以奈米金磁珠(Au/Fe3O4)結合抗體檢測特定病菌濃度及抗藥性之生物晶片
Development of a biochip using nano-Au/Fe3O4 particles and antibodies-covered to detect drug resistance of specific bacteria
摘要
近幾年來，由於抗生素的濫用，擁有抗藥性的病菌越來越多，使得醫生和研究人員不得不研發新型的抗生素來對抗病菌，然而在這場人類與病菌的戰爭是永無止境的，當務之急，就是減少抗生素濫用的情形，減緩病菌抗藥性的產生速度，才是上策。而盡早檢測出病菌有無抗藥性不但有助於醫師選擇適當的抗生素，也可以避免抗生素濫用的情形。本研究之目的為發展簡單、快速之檢測方式好取代早期檢測細菌及其抗藥性之方式，利用微機電製程技術製作用於實驗之微粒檢測的裝置晶片。此實驗大致分為四個部份：微電極的製作、奈米金磁珠的製作及分散、病菌收集計數量測、檢測病菌抗藥性可能性。其檢測方式主要是利用介電泳力的作用使溶液中欲檢測的病菌能夠收集於微電極的作用範圍內，提高計數的正確性及準確性。同時，本文也對奈米金磁珠的作用及在不同形狀電極下加入奈米金磁珠抗體混合病菌檢測之情形加以探討。 本實驗室利用射出成型、玻璃基材及CD光碟片製作出不同形狀的梳狀電極後，測量其結果。最後，再以梳狀電極進行病人檢體檢測，判斷有無待測病菌之存在，並探討其有無抗藥性。
並列摘要
Detection of drug resistance of bacteria at an early stage not only helps doctors in choosing the appropriate antibiotics, but also prevents antibiotic abuses. The purpose of this research is to develop a simple, efficient method for testing bacteria and detecting bacteria’s drug resistance, which can replace the traditional method. This research uses MEMS technology to construct the particle counting chip which will be used in the experiment. The experiment can be separated into four distinct parts: designing electrodes、fabricating and dispersing nano-Au/Fe3O4、cells collect and measure, and the detection of bacteria’s drug resistance. The detection method primarily uses the effect of DEP force to ensure that the bacteria in the solution can be collected within the range of electrodes, increasing the validity and accuracy of the count. Also, the text explores the effect of nano-Au/Fe3O4 particles and the bacteria’s reaction to the addition of nano-Au/Fe3O4 particles antibody under different shapes of electrodes. The research uses glass substrate and CD disc to produce different comb-like electrodes for measurement; results show that standing electrodes is most favorable for the experiment. Lastly, standing electrodes is used for testing the bacteria, indicating whether if the bacteria is drug-resistant.