含幾丁聚醣粒子溫感型共聚物

本研究首先將溫感型氮-異丙基丙烯醯胺(NIPAAm)單體及聚乙烯醇甲基丙烯酸酯(PEGMEA)單體，以雙氧水－維他命C作氧化還原自由基起始，合成不同組成比之溫感型共聚物水膠。我們將合成之共聚物與幾丁聚醣粒子混合，並以磷酸緩衝溶液(PBS)中為溶劑，配置不同重量百分比濃度(w/w%)之樣品。藉由倒瓶測試、DSC、穿透率測試，觀察其流體性質以及霧點(cloud point)。發現合成之poly(NIPAAm)其相轉換溫度為27℃；共聚物Poly(NIPAAm-PEGMEA)中的PEGMEA增加，其相轉換溫度可提升至36.4℃。樣品中若混有幾丁聚醣微粒，所測得之相轉換溫度並無重大差異。poly(NIPAAm)在緩衝溶液37℃時，一小時後之厚度變化約40%而poly(NIPAAm-pegmea)上升至80%，但加入幾丁聚醣之樣品厚度變化只有55%。MTT assay細胞活性測試發現所合成之高分子材料，並未發現會降低細胞存活率，隨著幾丁聚醣微粒添加量的增加，細胞存活率有上升的趨勢。

關鍵字

氮-異丙基丙烯醯胺；聚乙二醇；幾丁聚醣；溫感型共聚物；生物性密封劑

並列摘要

Poly(NIPAAm-PEGMEA) copolymers were prepared via free radical polymerization of various mole ratios of monomers initialized by H2O2-vitamin C redox initiators. The synthesized copolymers were further mixed with chitosan particles in phosphate buffered saline (PBS). The temperature dependences of flow property and cloud point of the solution with chitosan particles were investigated by the tube-invert, DSC, and light transmission tests. It was found that the poly(NIPAAm) could flow at 25oC, and formed a gel at higher temperature. The cloud point of the Poly(NIPAAm-PEGMEA) could increased to about 36.4oC with increasing of PEGMEA unit, but there had almost no change with addition of chitosan particles. The thickness of the poly(NIPAAm), and poly(NIPAAm-PEGMEA) hydrogels could increased to 40% and 80%, respectively, higher than that before swelling in PBS at 37oC. The ratio became only 50% for the poly(NIPAAm-PEGMEA)/chitosan particle system. The MTT assay indicated the biocompatibility could be improved by adding the chitosan particles into the copolymer hydrogel.

並列關鍵字

NIPAAm ； PEGMEA ； chitosan ； bio-sealant ； thermo-sensitive copolymer

參考文獻

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國際替代計量

含幾丁聚醣粒子溫感型共聚物

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