塑性鈣矽鋅骨混凝填補材之研究

本研究旨在開發一種塑性鈣矽鋅骨填補材料，並針對材料進行力學量測、結晶、表面、熱性質分析、離子釋出、細胞實驗等。此骨填補材是一種”無機－有機”複合材料，無機相的成分Zn0.075Ca2.925SiO5，是三鈣矽酸鹽 C3S (Ca3SiO5)參雜微量ZnO經由1400oC煆燒而得；有機相則是使用市售膠原蛋白，由於來源為牛隻，為避免植入體內時產生免疫反應，膠原蛋白都經過去免疫端化處理。　　三鈣磷酸鹽為工業用波特蘭水泥的主要成分之一，具有自固化特性，固化完成後則無法再塑型，而氧化鋅ZnO可抑止細菌繁殖並穩定C3S 結構，能夠大幅減少反應降溫時CaO之生成。添加膠原蛋白則可以增加材料的塑性，且具有海棉吸水的特性，能夠吸附組織液，不過缺乏力學強度，因此可與C3S之缺點互補。　　　　實驗方法主要是將Zn0.075Ca2.925Si3O5粉末混合NaH2PO4•2H2O溶液及膠原蛋白(collagen)製成之無機－有機系骨混凝填補材，並依照不同膠原蛋白添加量做為實驗變數依序為5、10、15、25、30、35、40(wt%)，根據實驗結果得知，添加膠原蛋白可讓材料的工作時間從未添加時的20分鐘大幅上升至45~70分鐘，pH值從11.5下降至7.5~8.2、細胞存活率從60%上升至80~95%、抗壓強度下降至26.5~7.5Mpa，使得基本性質有大幅改變，並具有以下特性:(1)高工作時間、(2)高度可塑性、(3)高抗壓強度，使用者可依照患部缺陷先進行塑型，再行填入，做為組織工程上之生物支架用途。

關鍵字

骨混凝填補材料；三鈣矽酸鹽；氧化鋅；膠原蛋白

並列摘要

This study aimed to develop deformable Ca-Si-Zn bone restoration complex, and focused on these analyses including mechanical properties, crystalline phases, surface properties, thermal analysis, ionic releasing and in vitro tests. This bone restoration complex is a type of “inorganic-organic” composite. The inorganic composition is Zn0.075Ca2.925SiO5, which was calcined at 1400oC by tricalcium silicate (Ca3SiO5) doped with ZnO. The organic composition is commercial deimmunized collagen. Tricalcium silicate(C3S) is one of the main composition of Portland cement. It provides the property of self-setting, after completely setting, deformation is unable. According to the previous studies, few ZnO doping in C3S could inhibit the formation of free CaO during preparation. ZnO not only stabilizes the structure of C3S but also provides antibiotic ability. In addition, collagen could increase plasticity of the composites and absorb tissue fluid and blood during wound healing. Zn0.075Ca2.925SiO5 was prepared through a solid-state reaction and then mixed with collagen and NaH2PO4•2H2O solution as buffer to obtain the complex. This bone restoration complex had a long working time, high plasticity and high compressive strength. So that, this bone restoration complex are able to be shaped in advance before application and served in tissue engineering as scaffolds.

並列關鍵字

Bone complex ； Restoration ； Tri-calcium silicate ； Zinc oxide ； Collagen

參考文獻

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Times Cited: 126 References: 37

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被引用紀錄

吳懿君（2012）。膠原蛋白/三鈣矽酸鹽複合骨膠之研究〔碩士論文，國立臺北科技大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0006-1308201214264500

延伸閱讀

王傳業（2007）。溶膠凝膠法製備之鈣矽骨水泥特性研究〔碩士論文，中山醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0003-1609200713473600
黃淑卿（2018）。骨修補及再生應用的矽酸鈣基生醫材料之特性分析〔博士論文，中山醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0003-0702201815295300
鄭人華（2006）。溶膠凝膠法製備鈣矽磷/幾丁聚醣複合材之特性研究〔碩士論文，中山醫學大學〕。華藝線上圖書館。https://doi.org/10.6834/CSMU.2006.00016
Kamadjaja, D. B., Harijadi, A., Soesilawati, P., Wahyuni, E., Maulidah, N., Fauzi, A., Ayu, F. R., Simanjuntak, R., Soesanto, R., Asmara, D., Rizqiawan, A., Agus, P., & Pramono, C. (2017). Demineralized Freeze-Dried Bovine Cortical Bone: Its Potential for Guided Bone Regeneration Membrane. International Journal of Dentistry, 2017(), 1-10-046. https://doi.org/10.1155/2017/5149675
Fu, Y. C. (2015). The study for combination of bioceramic and controlled release osteoinductive agents for bone repair [doctoral dissertation, Kaohsiung Medical University]. Airiti Library. https://doi.org/10.6832/KMU.2015.00117

國際替代計量

塑性鈣矽鋅骨混凝填補材之研究

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