電紡 collagen/chitosan/PVA 奈米纖維支架為傷口敷料之研究

Electrospun Collagen/Chitosan/PVA Nanofibers Scaffolds for Wound dressing

郭育仁(Yu-Jen Kuo)

指導教授 ： 林忻怡

國立臺北科技大學/工程學院/化學工程研究所/碩士(2011年)

https://doi.org/10.6841/NTUT.2011.00397

摘要

組織工程的重點在於細胞、支架、生長訊息。篇著重於支架的製造，支架要可以模仿天然細胞外間質，且要具有大量的孔隙，主要利用天然高分子及混合人工高分子，並利用電紡絲的方法，來製造出具有大量表面積及孔隙的結構，以模仿天然的細胞外間質。材料我們選擇膠原蛋白、幾丁聚醣及聚乙烯醇，皆是生物可分解材料，具有很好的生物相容性，並利用濃醋酸(90vol%)當成溶劑，因為可以有效降低表面張力，也可以增加電荷密度，以電紡絲的方式成奈米纖維，再藉由戊二醛蒸氣交聯，使機械性質提升，並用掃描式電子顯微鏡觀察表
面型態、傅立葉轉換紅外線光譜得知交聯後材料特徵吸收峰都還在，且三材料間可能會產生複雜的分子間吸以力、流變儀觀察流體的黏度、膨潤率、拉伸側詴測量其機械性質。且使用 MTT 測量細胞活性、並定量 DNA，測詴細胞與支架的生物相容性；細胞的結果顯示支架可能具有細胞毒性，此部分的清洗步驟可能還需更改。

關鍵字

電紡 ； 奈米纖維 ； 幾丁聚醣 ； 膠原蛋白 ； 戊二醛

並列摘要

Tissue engineering includes three major elements: cells, scaffolds, and signal molecules. We are focuses on how to prepare the mimic extracellular matrices whose components and porous structures are analogous to those of the native extracellular matrices (ECMs). Electrospun nanofibers have amazing characteristics such as very large surface area-to-volume ratio and high porosity with very small pore size. The process was an efficient technique for the fabricationof both natural and synthetic polymers nanofibers and various polymer have been electrospun. Collagen, chitosan, polyvinyl alcohol(PVA) are biodegradable, biocompatible materials. We using
concentrated acetic acid solution(90%) as a solvent. Because more concentrated acetic acid in water progressively decreased surface tension of the chitosan solution and concomitantly increased charge density of jet. Electrospun collagen/chitosan/PVA nanofibers were crosslinked by glutaraldehyde(GTA) vapor to enhanced stability. The morphology of the electrospun collagen/chitosan/PVA nanofiber scaffolds was observed by scanning electron microscopy (SEM) and stabilized by glutaraldehyde (GTA) vapor via crosslinking. Fourier transform infrared spectra analysis showed that the collagen/chitosan/PVA nanofibers scaffold do not change significantly, except for enhanced stability after crosslinking by GTA vapor. The thermal behavior was studied by thermal gravimetric analysis and the mechanical properties were Studied by tensile testing. To assay the biocompatibility of electrospun scaffolds, cellular behavior on the nanofibrous scaffolds was also investigated by SEM, methylthiazol tetrazolium testing(MTT) and dsDNA content. The cyototoxic effecy of GA-vapor crosslinked scaffolds was observed. The scaffolds show almosted no cellular activity.

並列關鍵字

electrospun ； nanofibers ； chitosan ； collagen ； glutaraldehyde

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