探討鼠傷寒血清型沙門氏菌及現行治療沙門氏菌的抗生Ceftriaxone 對於人類腸道上皮細胞β-防禦素表現之影響

Impacts of Salmonella enterica serovar Typhimurium and antibiotics on human β-defensin expression in human intestinal epithelium

陳姿伃(Tzu-Yu Chen)

指導教授 ： 方旭偉

國立臺北科技大學/工程學院/生物科技研究所/碩士(2012年)

https://doi.org/10.6841/NTUT.2012.00505

摘要

鼠傷寒沙門氏桿菌是引起全世界疾病和死亡的一種重要病原菌。至今，對於
鼠傷寒沙門氏桿菌，其感染人類腸道上皮細胞的早期免疫反應，所知仍然有限。
本研究首先建立LS174T 人類腸道上皮細胞體外培養模式，之後利用鼠傷寒沙門氏
桿菌(Salmonella Typhimurium)野生株SL1344，及缺少SPI-1 基因之突變株△spaS，
感染LS174T 後，以RT-PCR 及ELISA 方法，測量細胞激素IL-8、β-防禦素1、2
和3 之基因與蛋白質表現。而後，更進一步探討臨床上常用，治療鼠傷寒沙門氏
菌桿菌感染之抗生素Ceftriaxone，其對感染後細胞激素IL-8、β-防禦素1、2 和3
之基因與蛋白質表現之影響。
研究結果發現，野生株感染細胞後，可以增加細胞激素IL-8、β-防禦素1、2
和3 之基因表現，而缺少SPI-1 基因突變株，雖然也可以增加表現，但能力較野生
株差。野生株及突變株分別感染細胞後，以抗生素Ceftriaxone 處理細胞，可以抑
制IL-8 之基因表現，但不影響β-防禦素1 基因表現，相反的，卻增加β-防禦素2
和3 之基因表現。雖然Ceftriaxone 影響基因表現，但對於蛋白質表現影響並不顯
著。本研究成果指出，腸道細胞遭遇鼠傷寒沙門氏桿菌感染時，可能增加抗菌基
因表現協助抵抗感染，而治療鼠傷寒沙門氏菌桿菌感染時，投以抗生素Ceftriaxone
並不會干擾此抗菌機制。

關鍵字

沙門氏菌 ； 防禦素 ； 人類腸道上皮細胞 ； 抗生素

並列摘要

Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) is an
important pathogen which causes morbidity and mortality worldwide. So far, the early
immune responses in human intestinal epithelial cells after Salmonella Typhimurium
infection are little known. In this study, we have established an in vitro model to
observe IL-8、β-defensin 1、2 and 3 gene and protein expression in human intestinal
epithelial cells after Salmonella Typhimurium wild-type strain SL1344 or SPI-1 mutant
strain △ spaS infection by RT-PCR and ELISA assay . Furthermore, the effect of
Ceftriaxone treatment after infection on gene and protein expression of human IL-8、
β-defensin 1、2 and 3 were investigated.
Our results shown the mRNA level of IL-8、β-defensin 1、2 and 3 were increased
after wild-type strain and mutant strain infection. However, the ability of wild-type
strain is higher than mutant strain. The mRNA level of IL-8 after infection was reduced
by Ceftriaxone treatment. However, Ceftriaxone did not affect the mRNA expression
of β-defensin-1. On the contrary, the mRNA level of β-defensin 2 and 3 were
increased. Furthermore, the level of the protein expression affected by Ceftriaxone
treatment was not significant. The results of this study indicated that the intestinal
cells encounting typhimurium Salmonella infection may increase the antimicrobial
gene expression to resist infection, and treatment of Salmonella typhimurium
infection with Ceftriaxone may not interfere with this antibacterial mechanism.

並列關鍵字

Salmonellal ； β-defensin ； human intestinal epithelial cells ； antibiotics

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