- 學位論文
鈦基植體表面披覆氫氧基磷灰石之製備
The Fabrication and Bioactive Hydroxyapatite Layer on Ti and its alloys
摘要
本研究之實驗設計，以改善生物惰性之鈦與鈦合金(Ti and Ti-6Al-4V)之骨整合性(Osteointegration)。利用簡單之物理與化學方法之表面處理，以砂紙研磨(#600)、熱處理(400℃)與鹼性處理(5M/L 10M/L氫氧化鈉溶液)；增加鈦與鈦合金之表面特徵，改善材料表面積與離子濃度(OH group)。接著透過仿生浸鍍(Biomimetic immersion)之方式，浸鍍於人工模擬液體與過飽和鈣溶液，使鈦與鈦合金表面沉積生物活性之氫氧基磷灰石薄膜(Bioactivity hydroxyapatite layer)。 經過三十一天完整之鍍製週期，透過典型材料分析(SEM、EDX & XRD)、機械性質測試(Press tests)，分析氫氧基磷灰石薄膜之特性。結果指出，不同之鍍液所生成之球狀(Spherical)與板狀(Platy)磷灰石結構；其能譜儀結果指出其鈣磷比為1.67，與骨骼接近。且於X光繞射圖譜出現特性繞射峰(2 theta = 26°、32°)，驗證為氫氧基磷灰石晶態。附著力測試顯示，球狀磷灰石之薄膜附著力較板狀磷灰石佳。此外，為證實於鈦與鈦合金氫氧基磷灰石薄膜之生物相容性，經體外培養(In Vitro)三天之觀察，板狀結構有刺激細胞分化之可能性；然而，球狀磷灰石薄膜有較佳之存活率。 仿生浸鍍(Biomimetic immersion)，由不同鍍液所形成特性之氫氧基磷灰石薄膜於鈦與鈦合金表面，是可行的；於生物活性評估結果，顯著之細胞存活率，證實磷灰石薄膜之生物相容佳，相對提供於活體應用之參考。
並列摘要
The aims of this study is to develop method to improve the osteointegration of Ti and its alloys. Used simple chemical and physical methods of surface treatment that be polished with # 600 SiC papers, heat treated at 400℃ and alkali-treated with 5 M/L ,10 M/L NaOH aqueous solutions can increase the surface activity and OH ionic groups. Then, the biomimetic immersion were introduced into simulated body fluid and supersatured calcium solutions would produce a layer of bioactivity hydroxyapatite on Ti and Ti-6Al-4V. After 31days completed immersions periods, specimen were examined as Scan electron microscopy ( SEM ) , Energy dispersive of x-ray spectroscopy ( EDAX ) , and X ray diffraction. The results showed that the different solutions of SBF and SSCS help to form spherical-structure and platy - structure of apatite layer. EDX analyses indicated that Ca / P ratios are closed to human bone；the XRD patterns showed that character peaks occurred at about 2θ = 26°,32° which are identified as Hydroxyapatite crystal. In order to know adhesion strength between coating layer and Ti and Ti-6Al-4V. The press tests were demonstrated that the spherical structure of apatite have better bonding strength than that of the platy-structure apatite. However, in order to estimate the biocompatibility of Hydroxyapatite layer on Ti and Ti-6Al-4V to the osteoblast cell. In Vitro tests of three days were excuted. SEM morphologied demostrated that osteoblast cells on platy structure of apatite may be stimulated into cell differentiation stage. Therefore, the cell visibility of the osteoblast cells on spherical structure apatite were better than that of spherical-type. The methods of biomimetic immersion with different solutions formed special features of Hydroxyapatite layer on Ti and Ti-6Al-4V are possibility for fabrication. Therefore, the estimation of in vitro results has significant cell visibility , also confirmed the biocompatibility of apatite layer that can provide the evidences for basis in vivo.