探討生物添加分子合併白蛋白分子之潤滑機制

Investigation of the Lubrication Mechanism of Biomolecules Additives with Albumin Molecules

盧怡芳(Yi-Fang Lu)

指導教授 ： 方旭偉

國立臺北科技大學/工程學院/化學工程研究所/碩士(2013年)

https://doi.org/10.6841/NTUT.2013.00493

摘要

人工關節長期使用下會產生超高分子量聚乙烯(UHMWPE)磨耗顆粒，而導致人工關節鬆脫。由於潤滑液在人工關節對磨表面扮演很重要的角色，因此為了延長人工關節的壽命，需要從基礎上了解各分子間潤滑的機制。而人體潤滑液中成分有很多種，其中最為豐富的為白蛋白。
本研究利用pin-on-disc摩擦測試、圓二色光譜儀及標記白蛋白吸附實驗及流變性質，期望能瞭解熱變性白蛋白(HSA)與玻尿酸(HA)分子間交互作用。實驗結果得知，白蛋白玻尿酸摩擦係數並不隨著溫度而有所變化；同時也探討白蛋白構型變化對CoCrMo合金表面吸附行為的影響。由實驗觀察得知，熱變性白蛋白的摩擦係數與吸附量及α - helix結構損失具有正相關性。此外，我們更進一步探討白蛋白添加玻尿酸分子潤滑機制。結果顯示，當白蛋白及玻尿酸混合經過熱處理後，摩擦係數會降低，觀察其白蛋白構型變化不為主要影響摩擦係數的原因，而吸附量與黏度的增加為降低摩擦係數的原因。未來需進一步探討其他關節液成分與添加分子間的交互作用，以協助開發有效添加分子，以減少摩擦而改善潤滑，延長人工關節壽命。

關鍵字

鈷鉻鉬合金 ； 吸附 ； 生物分子 ； 白蛋白 ； 玻尿酸

並列摘要

UHMWPE wear debris could result in aseptic loosening of components after long-term use of artificial joint replacements. Lubricating fluid plays an important role in the contact mechanism of interfaces. Therefore, in order to extend the life of artificial joints, based on the need to understand the molecular mechanism of lubrication. Variety compositions of joint synovial fluid, among albumin is the most abundant protein.
In this study, investigated the lubrication behavior of thermal human serum albumin (HSA) and hyaluronic acid (HA) interaction by using pin-on-disc friction test and CD Spectroscopy and fluorescence isothiocyanate (FITC) labeled albumin adsorption and rheological properties. Experimental results single molecules system of albumin friction coefficient increased with temperature increased, HA friction coefficient independent temperature. We observed thermal denaturation of albumin adsorption amount, and α-helix structure loss lead to increase friction coefficient. In addition, we further explore HA added to albumin lubrication. Results show,as albumin and hyaluronic acid mixed after thermal treatment reduced friction coefficient. Albumin observed conformational changes influence friction coefficient is not the main reason. The Adsorption amount and viscosity increase lead to reduce friction coefficient.
In the future, we still need study lubrication mechanism between components of synovial fluid and additives. Assist to develop more effective lubricating of biomolecules additives lead to reduce friction and improve lubrication, in order to prolong lifetime of artificial joints.

並列關鍵字

CoCrMo alloy ； Adsorption ； Biomolecules ； Albumin ； Hyaluronic Acid

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