以毛細管電泳分離並使用線上濃縮技術來測量低濃度的玻尿酸含量

On-Line Concentration for Determination of Diluted Hyaluronic Acid by Capillary Electrophoresis

林凱鋒(Kai-Feng Lin)

指導教授 ： 黃玲媛

國立臺北科技大學/工程學院/化學工程研究所/碩士(2010年)

https://doi.org/10.6841/NTUT.2010.00407

摘要

本文使用毛細管電泳儀(capillary electrophoresis)來進行檢測玻尿酸(hyaluronic acid)的遷移行為，觀察玻尿酸在酸性和鹼性下的遷移情形情，並使用毛細管區帶電泳(capillary zone electrophoresis)來對不同濃度的玻尿酸進行觀察，並將所得的結果製成標準曲線來檢測一般市售的樣品，觀察市售樣品中玻尿酸含量；而實驗中也發現到使用毛細管區帶電泳的最低偵測濃度為0.4mg/mL無法在往下探測更低的濃度，為了解決這個問題，我們搭配了線上濃縮堆積的方式來使玻尿酸的偵測極限能夠更有效的改善，並偵測到更低濃度的玻尿酸，而線上濃縮的結果也成功的使玻尿酸的最低偵測濃度達到0.002mg/mL。
而本文中也嘗試著使用其他的分析模式，如:微胞電泳層析法、毛細管凝膠電泳來嘗試藉由界面活性劑所形成的微胞對不同分子量大小的玻尿酸做不同的選擇度和利用凝膠在管內形成不同大小的孔徑來分離不同分子量的玻尿酸，雖結果不如預期，但從其他分離成功的文獻中可以得知或許使用不相同的凝膠成分及不同濃度的組成，或許有成功分離的機會。

關鍵字

毛細管電泳分析儀 ； 玻尿酸 ； 毛細管區帶電泳 ； 微胞電泳層析法 ； 毛細管凝膠電泳 ； 線上濃縮

並列摘要

The study, we use capillary electrophoresis with on-line concentration to detect and to separate Hyaluronic acid. Hyaluronic acid has the different migration behavior in acidic and alkalinity buffer solution, respectively. Electrophoretic mobility of Hyaluronic acid is fast than the electroosmotic flow in the acidic buffer solution ,but it is opposite in the alkalinity. We use capillary zone electrophoresis to separate Hyaluronic and then fit a standard curve that using in commercial product. Using capillary zone electrophoresis to detect Hyaluronic acid is not perfect, because the limit of detection of Hyaluronic acid only 0.4mg/mL. We use the capillary zone electrophoresis with on-line concentration to solve the drawback. The on-lin concentration caused the limit of detection to 0.002mg/mL.
The other hand that we use micellar electrokinetic capillary chromatography and capillary gel electrophoresis to separate the different molecular of hyaluronic acid.
But the result is not perfect, but in orther literature has successful. Using other method or material maybe can successful to separate the different molecular of hyaluronic acid.

並列關鍵字

Capillary electrophoresis ； Hyaluronic acid ； Capillary zone electrophoresis ； Micellar electrokinetic capillary chromatography ； Capillary gel electrophoresis ； On-line concentration

參考文獻

sample stacking for separation and determination of cimaterol, clenbuterol

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