pH值對多酚化合物與硼酸鹽間結合常數的影響

pH Effect on Binding Constants for Polyphenols and Borates

余庭豪(Ting-Hao Yu)

指導教授 ： 黃玲媛

國立臺北科技大學/工程學院/化學工程研究所/碩士(2010年)

摘要

本實驗利用毛細管電泳儀經電流校正與黏度校正分析物的電泳遷移率，再計算多酚化合物(黃芩素;漢黃芩素;柚皮素;黃芩苷;芹菜素;木犀草素;原兒茶酸和對-香豆酸)與硼酸根離子間之結合計量比與結合常數，並使用紫外光/可見光光譜利用莫耳比法與Scott法來測定多酚化合物與硼酸根離子間之結合計量比與結合常數，並比較pH值對結合常數的影響。
結果顯示，紫外光可見光光譜法中的莫耳比率法與毛細管經黏度及電流校正後，酚酸與硼酸根離子間之結合計量比皆是1：2，表示毛細管電泳法與紫外光/可見光光譜法所得到的結果吻合。

關鍵字

多酚化合物 ； 類黃酮 ； 毛細管電泳法 ； 莫耳比率法 ； Scott法 ； 硼酸鹽 ； 結合常數 ； 結合化學計量比

並列摘要

In this article,we determine binding constants and binding stoichiometry for polyphenols compound (Baicalein、Wogonin、Naringenin、Baicalin、Apigenin、Luteolin、Protocatechuic acid,and p-Coumaric acid) and borates by capillary electrophoresis with viscosity correction,current correction and mole ratio method of ultraviolet-visible spectroscopic in different pH.
Compare the binding stoichiometry for polyphenols compound and borates by mole ratio method with the binding stoichiometry for polyphenols compound to borates by capillary electrophoresis with viscosity correction and current correction, we find no matter which kind of methods,it shows good agreement with 1：2.

並列關鍵字

Polyphenols compound ； Flavonoid ； Capillary Electrophoresis ； mole-ratio method ； Borate ； Binding constants ； Binding Stoichiometry

參考文獻

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