雙胺基生物分子表面官能化之單壁奈米碳管作為基因載體之研究

The study of diamine biomolecule -functinolized single-walled carbon nanotube as vector for gene delivery

李佳育(Chia-Yu Li)

指導教授 ： 許淙慶 ； 林峰輝

國立臺北科技大學/工程學院/材料科學與工程研究所/碩士(2007年)

https://doi.org/10.6841/NTUT.2007.00264

摘要

無資料

關鍵字

基因載體 ； 單壁奈米碳管 ； 雙胺基生物分子 ； 細胞毒性

並列摘要

The development of new and stable gene carrier is of great importance to improve the tranfection
efficiency and achieve to certain fundamental needs in gene therapy such as therapeutic protein
formation, immunogenicity enhancement and apoptosis induction. The stability and applicability
of the system, which contains single-walled carbon nanotube (SWNCT) to combine with certain
sequence of plasmid DNA (EGFp-C1), was examined by computer simulation. Raman
spectroscopy was used as supporting information to verify the process and phenomenon of
complex formation. Two diamine molecules, 1,4-diaminobutane and polyoxyethylene bis-amine,
were applied in order to load positive charges onto the surface of SWCNT under physiology
condition and also enhance the binding ability of CNT to EGFp-C1. The formation of peptide
bond was examined with FTIR spectroscopy to confirm the result of crossliking. Concentration
of surface functional groups was checked with TGA, which found that they are at an extremely
low concentration but show great influence on the physical property. Full-scale cell compatibility
was evaluated through LDH, MTT, Total DNA and IL-6 release. The binding efficiency of
functionalized SWCNT to EGFp-C1 was analyzed through the binding strength under
electrophoresis. The cell viability assay found that functionalized SWCNT show no LDH release
and increase the mitochondrial activity. The release of IL-6 indicates protrusion of SWCNT
through cell membrane as possible mechanism. Gene expression for in-vitro transfection was
examined by fluorescence microscopy.

並列關鍵字

gene carrier ； single-walled carbon nanotube ； 1,4-diaminobutane ； polyoxyethylene bis

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