塗佈不同型態的膠原蛋白奈米纖維修飾Ti6Al4V鈦合金表面對骨母細胞培養之影響

由於鈦合金具有良好的生物相容性，因此被廣泛使用於骨折固定、人工牙根、人工關節、人工骨骼、牙齒矯正線與血管支架等臨床應用。本研究將Ti6Al4V鈦合金進表面塗佈膠原蛋白奈米纖維，以期能進一步提升材料之生物相容性。本研究以不同的電紡方式在金屬表面塗佈高密度隨機COL(H)、低密度隨機COL(L)、高密度順向COL(A)電紡纖維，並進行物理及生物相容性測試。從物理測試結果可得知高密度隨機與高密度順向組的表面裸露的金屬比例約為1%，而低密度電紡組則有15%至25%為裸露的金屬。本研究在COL(H)、COL(L)及COL(A)三組樣本表面培養7F2骨母細胞進行生物相容性測試。我們將進行I型膠原蛋白質定量測試、鹼性磷酸酶活性測試及鈣礦化測試以了解骨母細胞分化的程度，並由DNA定量了解細胞增生的趨勢，再以掃描式電子顯微鏡觀察細胞型態。實驗結果顯示骨母細胞在COL(H)、COL(L)及COL(A)三種樣本表面皆能貼附，並呈現扁平梭狀的型態，且在順向電紡表面的細胞會沿著纖維的方向伸長成梭狀，而高密度及低密度隨機電紡表面的細胞則會往任意方向生長。培養三週後，細胞在三組樣本上的細胞增生、I型膠原蛋白分泌量、鹼性磷酸酶活性測試及鈣礦化測試上皆大致呈現相同趨勢，沒有觀察到明顯差異。

關鍵字

骨母細胞；膠原蛋白；奈米纖維； Ti6Al4V鈦合金

並列摘要

Since titanium alloy has good biocompatibility, it is widely used in fracture fixation, dental implant, artificial joints, artificial bones, teeth straightening, vascular stents and other clinical applications. In this study, we successfully fabricated three kinds of collagen-coated Ti6Al4V (Ti64) surface -the high-collagen fiber density (COL-H) surface, the low-collagen fiber density (COL-L) surface, and the aligned high-collagen fiber density (COL-A) surface. EDX results showed that high fiber density surfaces had about 1% metal exposed on the surface while low fiber density surfaces had approximately 15~25% bare metal surface area. Scanning electron microscopy examination showed that osteoblast cells attached to all three surfaces and had shuttle-like flat patterns . Cells on COL(H) and COL (L) elongated in all directions , while cells on COL(A) elongated in the direction of the alignment of the fibers. In the first two weeks, cell on COL(H) proliferated less and secreted less type I collagen than those on COL(A) and COL(L). However, the three groups have no significantly different on type I collagen secretion test, cell proliferation, ALP activity test and calcium quantitative test.

並列關鍵字

Osteoblast ； Collagen ； nanofiber ； Ti6Al4V

參考文獻

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2. Zhu X, Chen J, Scheideler L, Reichl R, Geis-Gerstorfer J, effects of topography and composition of titanium surface oxides on osteoblast responses, Biomaterials, 2004, 25:p4087-4103

3. T.C.A. Phan, J. Xu and M.H. Zheng, Interaction between osteoblast and osteoclast impact in bone disease, Histol Histopathol ,2004, 19:p1325-1344

4. Okazaki et al., Cytocompatibility of various metal and development of new titanium alloys for medical implants, Materials Science and Engineering: A,1998, 243: p250-256

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塗佈不同型態的膠原蛋白奈米纖維修飾Ti6Al4V鈦合金表面對骨母細胞培養之影響

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