磁性明膠/磷酸鈣奈米球之製備分析及作為癌症熱療之研究

The Preparation and Characterization of Magnetic Gelatin/CaP Nanospheres as Thermoseed for Cancer Hyperthermia

詹子毅(Zih-Yi Jhan)

指導教授 ： 許淙慶 ； 林峯輝

國立臺北科技大學/工程學院/材料科學與工程研究所/碩士(2009年)

摘要

溫熱療法為目前在癌症治療上熱門的研究方向，癌細胞對熱極為敏感，當溫度達到43℃以上時，癌細胞即會死亡。若將磁性奈米顆粒，放置在癌細胞周圍並且外加交流磁場，則磁性奈米顆粒會因磁滯效應而釋放出熱能，當癌細胞週圍溫度升至43℃以上時，即可殺死癌細胞，此治療方法稱為感應式溫熱療法。

磷酸鈣為人體骨骼、牙齒主要的無機物成份，為無毒性、可生物降解、具生物相容性，本研究在製備磷酸鈣的過程中，藉由摻雜二價鐵離子，取代鈣離子的位置讓磷酸鈣具有磁性，並導入具有多種官能基、無毒性、可生物降解、具生物相容性的高分子-明膠，以期讓後續接枝製程更順利。研究製程使用逆微胞法 - 多庫酯鈉、正己烷、水三相系統製備具有磁性的磷酸鈣明膠奈米顆粒，期望將來有機會應用於癌症感應式溫熱療法。

關鍵字

磷酸鈣 ； 明膠 ； 逆微胞法 ； 溫熱療法 ； 離子替換 ； 磁滯損失

並列摘要

Hyperthermia cancer treatment as therapy is popular in biotherapy field. Cancer cells are sensitive to heat. As temperature rise to 43~46℃, the heat treatment will cause cancer cells death. When magnetic nanoparticles is placed around cancar cells at an AC magnetic field, hysteresis loss will make temperature rise to 43℃ then kill cancer cells. The method is called magnetic hyperthermia.
Calcium phosphate ( CaP ) is main mineral of bone and teeth, which is non-toxic, biocompatibility and biodegradable. In this study, the Fe2+ is doped into calcium phosphate to substitute Ca2+. Iron calcium phosphate ( calcium phosphate doped iron Fe( II ) ) can release heat in alternating magnetic field because it has magnetism. In the preparation process, gelatin is added because it has a lot of functional groups.
In this study, iron calcium phosphate-gelatin nanoparticles is prepared by reverse micelle, using three phases system: n-hexane, AOT, water, and temperature is set at 25℃ while pH is set at weak acid around 5. CaP-Fe/gelatin nanoparticles is placed at an AC magnetic field, the heat is liberated due to hysteresis loss. It is believed that CaP-Fe/gelatin nanoparticles is one of the most potential materials in magnetic intracellular hyperthermia in the future.

並列關鍵字

calcium phosphate ； gelatin ； cancer hyperthermia ； reverse micelle ； ion substitute

參考文獻

[3] Hanahan D, Weinberg RA, “The hallmarks of cancer”, Cell, vol.100, no.1, 2000, pp.57-70

[6] O. S. Nielsen, M. Horsman and J. Overgaard,” A future for hyperthermia in cancer treatment”, Eur. J. Cancer, vol.37, 2001, pp.1587-1589

[7] K. Overgaard and J. Overgaard, ” Investigations on the possibility of a thermic tumor therapy”, Eur. J Cancer, vol.8, 1972, pp.65-78

[8] S. Mornet, S. Vasseur, F. Grasset and E. Duguet, "Magnetic nanoparticle design for medical diagnosis and therapy," J. Mater. Chem., vol.14, 2004, pp.2161-2175

[9] P. Moroz, S. K. Jones, B. N. Gray, “Status of hyperthermia in the treatment of