探討改質界面上特異性蛋白質吸附影響之研究

The influence of specific protein adsorption on the modified interface

蔡介逸(Jie-Yi Tsai)

指導教授 ： 陳慶國

國立臺北科技大學/工程學院/生化與生醫工程研究所/碩士(2014年)

https://doi.org/10.6841/NTUT.2014.00646

摘要

本研究欲了解蛋白質接枝後在膜上與其他蛋白質吸附的交互作用，首先透過11-Mecreaptoundecanoic acid對裸金晶片進行化學性的修飾處理觀察晶片蛋白質的吸附能力，以QCM做為質量感测儀器。由於碳氫鏈間的凡得瓦力以及金-硫間的共價鍵結，使得分子在金表面形成單分子有規則的自組裝膜( self-assembled monolayer，SAM)。利用EDC/NHS修飾改質表面醋酸根末端來增加蛋白質被醋酸根接合的數量，透過Anti-BSA與蛋白質BSA和Ig G間的交互作用顯示，注射蛋白質BSA與Ig G後沒有明顯的頻率下降，說明在此Anti-BSA蛋白質膜層上是非特異性吸附現象。藉由電化學阻抗譜（electrochemical impedance spectroscopy，EIS)測定接枝過後的晶片與裸金晶片作比較，的確在金表面形成一層薄膜而產生阻抗的訊號圖。藉由EIS的等效電路模擬圖譜觀察金表面改質的電阻變化來推斷改質效果以及更近一步的應用。

關鍵字

石英晶體為天秤 ； 晶片改質 ； 單分子自組裝膜 ； 交流電阻抗

並列摘要

After this study was to understand the interaction of proteins in the membrane graft and other protein adsorption, first through a 11-Mecreaptoundecanoic acid on bare gold wafer chemically modified protein adsorption capacity of processing wafers observed to QCM as a mass sensing instruments . Since the van der Waals forces between the hydrocarbon chains and gold - a covalent bond between the sulfur, making the molecules self-assembled monolayer on gold surface of single molecules have rules (self-assembled monolayer, SAM) formation. Using the EDC/NHS-modified surface modified to increase the number of end acetate acetate is bonded to the protein through the interaction of BSA Anti-BSA and Ig G and protein between the display, after the injection of BSA and Ig G protein no significant reduction in the frequency, instructions on this Anti-BSA film nonspecific protein adsorption phenomena. By electrochemical impedance spectroscopy (electrochemical impedance spectroscopy, EIS) determine the graft after the wafer as the wafer and the bare gold compare the impedance signal is indeed generated in gold surface to form a film. With the change in resistance equivalent circuit of the analog spectrum observed gold surface modification of the EIS to infer modification effect and closer applications.

並列關鍵字

QCM ； surface modified ； self-assembled monolayer ； EIS

參考文獻

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