多酚化合物與硼酸鹽間結合常數的測定

Determination of Binding Constants for Polyphenols and Borates

張雅婷(Ya-Ting Chang)

指導教授 ： 黃玲媛

國立臺北科技大學/工程學院/化學工程研究所/碩士(2009年)

摘要

本研究利用經電流校正與黏度校正分析物電泳遷移率的毛細管電泳法以及使用紫外光/可見光光譜利用連續變動法之Job圖與Scott法來決定多酚化合物與硼酸根離子間之結合計量比及結合常數。結果顯示毛細管電泳法與紫外光/可見光光譜法所得到的結果大致吻合；黃芩(Baicalin)、漢黃芩素(Wogonin)、芹菜素(Apigenin)、木犀草素(Luteolin)與柚皮素(Naringenin)與硼酸根離子間之結合計量比皆是1：2。以毛細管電泳法結果所計算得到之多酚化合物與硼酸根離子間之結合常數分別如下，黃芩素(Baicalein)為1.64×105、Baicalin為7.37×104、Wogonin為2.10×105、Apigenin為1.51×105、Luteolin為1.93×105、Naringenin為1.94×105、粗毛豚草素(Hispidulin)為7.40×104、Protocatechuic acid為3.27×107、對-香豆酸( p-Coumaric acid)為1.16×108；顯示酚酸化合物與硼酸根離子間之結合常數遠大於類黃酮化合物與硼酸根離子間之結合常數。

關鍵字

毛細管電泳法 ； Job法 ； 多酚化合物 ； 類黃酮 ； 硼酸鹽 ； 結合常數 ； 結合化學計量比

並列摘要

In this article, we determine binding constants and binding stoichiometry for polyphenols compound and borates by capillary electrophoresis with viscosity correction , current correction and job’s method of ultraviolet-visible spectroscopic. Compare with the binding stoichiometry for polyphenols compound and borates by Job’s method,binding stoichiometry for polyphenols compound (Wogonin、Luteolin、Apigenin、Naringenin ) to borates by capillary electrophoresis with viscosity correction and current correction show good agreement with 1：2 , which is the same as the result by Job’s method. The binding constant of polyphenols compound and borates：Baicalin (1.64×105)，Wogonin(2.10×105)，Luteolin(1.93×105)，Apigeni(1.51×105)，Naringenin(1.94×105)，Hispidulin(7.40×104)，Protocatechuic acid (3.27×107)，p-Coumaric acid(1.16×108). So, the binding constant of polyphenols compound and borates is bigger than far flavonoids compound and borates.

並列關鍵字

Capillary Electrophoresis ； Ultraviolet-visible spectroscopic ； Polyphenols compound ； Flavonoid ； Borate ； Binding constants ； Binding Stoichiometry

參考文獻

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