- 學位論文
生物添加分子在人工關節材料表面之潤滑機制與交互作用
Lubrication mechanism and interactions of biomolecular additives on the artificial joint material surfaces
摘要
目前最常見的人工關節對磨材料組合為超高分子量聚乙烯(UHMWPE)對 鈷 鉻 鉬 合 金 (CoCrMo aolly) 。 UHMWPE 之 磨 耗 顆 粒 會導致 骨溶解現象 (osteolysis)，造成人工關節鬆脫。而潤滑液存在於人工關節對摩材料間中可降低 元件間之摩擦及磨耗，先前研究中小牛血清中添加含有4.5mg/mL藻酸鈉(AA)與 4.5mg/mL角叉菜膠(CRG)對人工關節材料最具顯著的潤滑效果。因此本實驗將探 討AA及CRG對蛋白質間之潤滑機制並調控AA及CRG之不同濃度組合進行摩擦 力測試。 本研究利用pin-on-disc摩擦及往復式磨耗測試機，探討AA及CRG兩種生物添 加分子不同濃度之組成對人工關節對磨材料之磨潤行為，實驗結果發現，當牛血 清中同時含有AA與CRG兩種生物添加分子相互混合後其摩擦係數皆比單成分 CRG來的低，目前牛血清中含有2.25mg/mL AA與12.5mg/mL CRG為最具顯著潤 滑效果，同時此潤滑液配方亦可降低磨耗量。接著更進一步利用石英晶體微天平 (QCM)與流變性質，探討其潤滑機制。由實驗結果得知，黏度變化不是影響摩擦iii 係數的主要原因；推測可能是分子之吸附行為造成AA及CRG存在於小牛血清中 摩擦係數降低的原因。未來可在利用螢光染色之方法進一步證明並使用人工關節 模擬器根據ISO標準進行測試並加以驗證，將此嘉惠使用人工關節的病患且延長 人工關節壽命。
並列摘要
The most common artificial joint material is ultra-high molecular weight polyethylene (UHMWPE) and Cobalt-Chromium-Molybdenum alloy (CoCrMo alloy). UHMWPE wear debris could lead to osteolysis and that result in loosening and thus failure of the implant. Periprosthetic fluid presents between artificial joint surfaces and acts as a lubricant which prevents wear and reduces friction. From previous studies, the most significant combination of lubricant composition is bovine calf serum containing 4.5mg/mL of alginic acid and 4.5mg/mL of carrageenan. Therefore, our experiment will investigate lubrication mechanism of alginic acid and carrageenan between proteins and adjust alginic acid and carrageenan different combinations of concentrations to do friction test. In this study, pin-on-disc friction test and reciprocating wear testing machine were carried out to investigate two kinds of biomolecules additives to change different combinations of concentrations on friction and wear behavior of artificial joints. We observed when bovine serum containing both alginic acid and carrageenan, the coefficient of friction is lower than carrageenan. The most significant combination of lubricant composition is bovine calf serum containing 2.25mg/mL of alginic acid and 12.5mg/mL of carrageenan. And this lubricant formulation also reduces the wear. And then investigate two biomolecules additives alginic acid and carrageenan between protein molecules interactions by using rheological properties and quartz crystal microbalance. The results show that viscosity is not the main reason to affect the coefficient of friction; we surmise the adsorption behavior of molecules is resulting the friction coefficient decrease. In the future, we can use the FITC investigate the lubrication mechanism , and artificial joint simulator testing in accordance with ISO standards and verified, this benefit in patients with artificial joints and prolong the life of artificial joints.