- 學位論文
以動物模型探討生物添加分子對骨溶蝕現象之影響
The effects of biomolecular additives on particle-induced osteolysis an in-vivo animal study
摘要
人工關節中，因為超高分子量聚乙烯墊片與鈷鉻鉬合金對磨產生許多磨耗顆粒，顆粒被巨噬細胞吞噬時，會使巨噬細胞釋出發炎因子而活化蝕骨細胞，導致骨溶蝕現象，而使人工關節脫落。 因此如何減少磨耗以減少骨溶蝕現象的產生，將是人工關節重要的課題，所以本研究的目的是探討先前實驗室所研究出的生物添加分子在不同濃度下，對骨溶蝕現象的影響。 首先，在大鼠的膝關節打入生物添加分子，發現生物添加分子在大鼠膝蓋並未產生明顯發炎，再利用半導體製程中的微影蝕刻製作切割裝置，大量製造出次微米UHMWPE顆粒，以次微米磨耗顆粒混合不同濃度之生物添加分子誘導出小鼠顱骨的骨溶蝕現象，最後觀察其骨溶蝕現象的程度，發現添加0.05%及0.1%的維他命E，並未明顯減少小鼠顱骨的骨溶蝕現象，而添加高濃度的藻酸鈉及角叉藻二醣，會使小鼠顱骨的骨溶蝕現象有顯著的下降。
並列摘要
Ultra-high molecular weight (UHMWPE) and CoCrMo alloy produce many wear particles in the artificial joints. When particles are engulfed by macrophages, macrophages will release inflammatory factors to activate osteoclasts. Too many osteoclasts will lead to osteolysis and make artificial joints loosen. Therefore, it is important to reduce wear particles to decrease osteolysis. The research purposes to investigate that biomolecular additives in different concentration affects osteolysis. First, we injected biomolecular addictives into knees of rats. We found biomolecular addictives didn’t induce the significant inflammatory. Next, we used lithography and etching in the process of the semiconductor to produce cutting device which can make many submicron UHMWPE particles. Mixing submicron wear particles with the biomolecular addictives in the different concentration induced osteolysis in the calvarial bone of the mouse. Last, we observed the level of osteolysis. We found adding 0.05% and 0.1% Vitamin E isn’t effective in decreasing osteolysis. Adding alginates and carrageenans in high concentration is effective in decreasing osteolysis.