硝基還原酶螢光探針的合成路徑探討

Exploration of synthetic routes for Nitroreductase fluorescent probe

王辰安(Chen-an Wang)

指導教授 ： 黃聲東

國立臺北科技大學/工程學院/生化與生醫工程研究所/碩士(2014年)

https://doi.org/10.6841/NTUT.2014.00814

摘要

硝基還原酶(NTR)是細菌中一種已知的黃素酶，可以催化硝基芳香化合物形成胺基芳香化合物的還原反應，硝基還原酶被廣泛使用生物醫藥、抗菌劑等，也可以應用在檢測含硝基芳香化合物的環境汙染物。檢測硝基還原酶的方法很多，常見的是利用螢光分子探針，但多數螢光探針是溶解性較差，因此設計一個溶解性較好的新隱藏式螢光探針NBF-L來即時偵測硝基還原酶的活性，隱藏式螢光探針在未活化可以穩定存在，僅在特一的化學反應有強烈的螢光釋出，且為不可逆的反應，但是在合成隱藏式螢光探針NBF-L的過程中，結果不如預期，從此進行研究探討。

關鍵字

長波長螢光探針 ； 硝基還原酶

並列摘要

Nitroreductase (NTR) is a flavoenzyme with β-nicotinamide adenine dinucleotide (NADH) or β-nicotinamide adenine dinucleotide phosphate (NADPH) as a cofactor. NTR catalyzes the reduction of nitro compound to correspond in amine. The use of fluorescent probes assists real-time detection of NTR for hypoxia. We prepare a new long-wavelength latent fluorogenic probe, NBF-L, for the monitoring NRT activities. The fluorogenic probes are stable that unmask their intense fluorescence only by a user-designated chemical reaction, and they are especially useful tools for basic research in the biological sciences. The fluorogenic chemical transformation of NBF-L triggered by NRT in the presence of NADH is through a series of tandem reaction which are irreversible in aqueous media. But in the synthesis of latent fluorescent probes NBF-L have some problems and discuss these problems.

並列關鍵字

long-wavelength probe ； Nitroreductase

參考文獻

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