以奈米金粒子結合抗體檢測特定生物微粒之生物晶片

Development of a Biochip Using Nano-goldparticles and Antibodies-covered to Detect Specific Bioparticles

林郁桓(Yu-Huan Lin)

指導教授 ： 黃榮堂 ； 施勝雄

國立臺北科技大學/機電學院/製造科技研究所/碩士(2007年)

摘要

本研究主要是利用微機電製程技術製作微粒檢測的裝置晶片，此晶片大致分為兩個部份：（1）微電極的製作（2）細胞的收集與量測。主要是利用介電力的作用使溶液中欲檢測的粒子能夠收集於微電極的作用範圍內，使得計數的正確性及準確性提高。此方法特徵為使用一晶片，晶片上設有交錯梳狀電極，梳狀電極上設一儲槽，其施行步驟包含：在試管中加入檢體以及對欲檢出目標生物微粒具專一性的結合奈米金的抗體，充分混合後，使目標生物微粒與該對應的奈米金抗體接合；汲取定量的混合液，滴入晶片上的儲槽；對梳狀電極施以一特定範圍頻率的交流電訊號，使所有結合上奈米金抗體的生物微粒，因為介電泳(DEP) 的作用力，能有效吸附於交錯梳狀電極的邊緣；加入清水，傾倒出混合液，重覆多次，直到清除檢體，此時仍維持對梳狀電極施以一特定範圍頻率的交流電訊號；將梳狀電極再接上等效電路與示波器，量取梳狀電極間的阻抗，尤其是電容值，並與空白電極之對照組比較，其差異值與電極上吸附的生物微粒的數量成正比。

關鍵字

微機電製程技術 ； 介電力 ； 奈米金粒子

並列摘要

In this paper, we use MEMS technology to produce particle counting chip witch includes trapping electrode and cells collect and measure. Let particles drop in chip inlet then use dielectrophoresis(DEP) force to concentrate and induce particles. The method employs a substrate with interlaced comb-like electrodes on which an amount of sample mixed with antibodies-covered nano-gold particles is dropped. Then the alternative signal with specific frequency band applies on the comb-like electrodes such that under DEP force the Au-modified bioparticles can separate from the sample and be absorbed effectively onto the edges of the electrodes. After rinsing with water and pouring out the residual sample several times, the absorbed bioparticles on the edges of the electrodes will be measured by equivalent circuit and Oscilloscope. The measured capacitance deviation comparing with a reference empty comb-like electrodes will reflect the amount of the absorbed bioparticles.

並列關鍵字

MEMS ； dielectrophoresis(DEP) ； nano-gold particle

參考文獻

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