- 學位論文
雷射顯微摘取用多層轉移膜之研究
The Study of Multi-Layer Transfer Film for Laser Capture Microdissection
摘要
本研究主要是雷射顯微摘取(Laser Capture Microdissection, LCM)多層轉移膜之開發。由於之前開發單一材質之膜厚為30μm之熱熔膜，已成功應用於雷射顯微摘取(LCM)系統上，但本研究期望能再降低熱熔膜之厚度應用於LCM系統上，因此利用支撐層、光學多層膜此兩種材質與利用MI值不同之EVA溶液之兩種方式開發出新的熱熔膜，以作為雷射雷射顯微摘取(LCM)系統之使用。 實驗使用乙烯醋酸乙酯(Ethylene Vinyl Acetate, EVA)、聚酯薄膜(polyester, PE)、TiO2與 SiO2分別為熱熔膜、支撐層與光學多層膜之主要材料，經由調配MI值不同的EVA溶液、基板製備與旋轉塗佈等製程，以完成各種不同厚度的熱熔轉移膜，而熱熔膜的評價是使用原子力顯微鏡(AFM)、掃描式電子顯微鏡(SEM)、雷射顯微摘取(LCM)系統等，找出最佳的表面粗糙度、熱熔深度、不同膜厚與熔點大小之關係，以提供雷射顯微摘取系統於不同目標組織切片之應用。
並列摘要
The purpose of the study is to provide the development of multi-layer transfer film for laser capture microdissection. Before developing single material of the transfer film thickness of 30 μm, it has been successfully applied to the LCM system. In the study, we hope could reduce the film’s thickness for LCM system, and uses support layer, multi-layer optical film and uses EVA solution of two melt index values to develop new hot-melt film for the usage of LCM system. By using EVA, polyester film, TiO2 and SiO2 as hot-melt film, the support layers, and major multi-layer optical materials. By way of mixing different MI value EVA solution, substrate preparation, and spin coating etc., we made various hot-melt film with different thickness. In this study, hot-melt film’s appraisal which used AFM, SEM, and LCM system, and found the best surface roughness, hot-melt depth, and different thickness and size of the melting point to provide LCM system in different sections of the target application.