- 學位論文
頻閃式光學映射系統之研製
Development of Strobscopic optical mapping system
摘要
光學映像系統，為研究心臟電生理之儀器，本研究將電壓敏感性染劑添加於心臟，在照射能量於心臟表面後激發螢光，心臟細胞離子濃度伴隨電壓變化使螢光光譜產生飄移，螢光透過光學系統後成像於相機，利用記錄心臟影像光強變化對映之細胞內離子流動。由於心臟離子流動及傳導非常快速，需要用高速擷取影相設備，方能記錄短時間內之變化，因此，在高速取像的限制條件下，傳統光學映像系統無法有細緻的空間解析度，且售價昂貴。本研究提出頻閃技術，利用心臟動作電位重複之特性，解決高速取像之使用限制，使系統擁有大範圍之時間與空間解析度，利於心臟動作電位傳導之研究。另一方面，心臟電生理的生理數據整理及統計上，相關程式設計及開發方面的研究依然十分缺乏，現有的系統在使用上，局限於其所開發的軟體，後端使用者無法根據其所需變化精進。因此，本研究開發一套光學式影像映像系統，目的於降低其系統造價，搭配頻閃技術調配控制影像擷取器取像，研發高時間與空間解析度及即時影像的影像映像光學硬體系統，並將我們所研發的光學影像映像系統用於觀察及研究小鼠心臟生理特性研究上。
並列摘要
Optical Mapping is one of instruments in the field of cardiac electrophysiolog. The measurement is performed by firstly injecting electro-fluorescent dye into the heart tissues. Surface of the heart tissues emit fluorescent light when there is a action potential change and the emitted light is then captured by camera through an imaging system. The distribution and flow of the action potential on the surface of the heart tissues are evaluated from the captured images by analyzing variation and direction of the intensity variation in time and spatial domain. The electric current flow inside the tissue is very fast, therefore high speed imaging system must be employed and the phenomenon can only be recorded in a short period of time. The drawback is the spatial resolution has to be sacrificed and the price of the optical mapping systems is very high. The objective of this research is to build an optical mapping system with reasonable price without having to sacrifice the time and spatial resolution. The developed optical mapping system is equipped with stroboscopic lighting which is synchronized with the image acquisition system and also a real-time image acquisition system. Stroboscopic lighting is employed to overcome the limitation of camera speed by recombining the captured periodic optical signal. Therefore, the developed system possesses high resolution in both time and space compared to the conventional optical mapping system. Heart tissue of an experimental rat is employed in the experiment to evaluate the feasibility of the proposed method.