PPC/PLA/TCP 復合材料之特性分析與探討對骨癒合的影響

THE CHARACTERISTIC OF PPC/PLA/TCP COMPOSITE SCAFFOLD AND EFFECTS ON BONE HEALING

高暐矞(Wei-Yu Kao)

指導教授 ： 陳瑞明

臺北醫學大學/醫學院/醫學科學研究所/碩士(2013年)

摘要

骨折 (bone fracture) 是現在人長發生的意外，亦是骨質疏鬆症 (osteoporosis)
患者跌倒後常發生的病症。進幾年，組織工程 (tissue engineering) 研發生物材料
(biomaterials) 提供足夠的空間與時間讓骨癒合 (bone healing) 以作為治療骨缺
陷的支架 (scaffolds)。目前已證實碳酸丙烯酯 (polypropylene carbonate , PPC)具
有自然降解的特性，然而，PPC 非天然聚合物，生物相容性較差。本研究目的在
提升PPC 的生物相容性，利用複合材料的技術，將聚乳酸 (poly-lactic acid, PLA)
與生醫陶瓷 (tricalcium phosphate, TCP) 加入PPC 中， 利用鹽析法製得
PPC/PLA/TCP 複合材料支架 (PPC/PLA/TCP composite scaffold, PPTCS)。在水接
觸角 (water contact angle) 的實驗結果發現，加入 2 % 的PLA 與 8 % 的TCP
組別中，水接觸角顯著地下降；為了提高生物相容性，利用鹽析法製得的PPTCS
經實驗測量，發現具有均勻分布的孔洞且孔洞直徑約為 111.12 μm。我們利用骨
母細胞 (osteoblasts) 佐證細胞會貼附在PPTCS 且隨著天數的增加而生長，顯示
PPTCS 具生物相容性。為進一步研究PPTCS 材料之應用性，利用動物模式在小
鼠大腿骨施與直徑 1 mm 損傷並填入PPTCS，犧牲後利用組織病理切片與微型
電腦斷層掃描 (micro-computed tomography; micro CT) 評估是否造成肝毒性與
骨癒合情況。結果發現，PPTCS 組別的肝組織並無纖維化或皺縮，且骨小樑
(trabecular bone number) 及骨小樑密度 (trabecular bone connectivity density) 與
骨缺陷組別相比都有上升的趨勢，且不會對皮質骨 (cortical bone) 的癒合產生影
響。綜合以上結果可知，PPTCS 是具有均勻分布的孔洞及生物相容性的生醫支
架，無肝毒性且能促進骨的癒合。當前，全世界生物材料的產值已超過40 億美
金，因此，將PPTCS 作為幫助骨再生的生物材料具有相當的潛力。

關鍵字

骨癒合 ； 生醫材料 ； 複合材料

並列摘要

Bone fracture often occurs in accident, and it is a common disease that happens
in osteoporosis patients. Recent years, the biomaterials are applied to bone
regeneration in tissue engineering. The biomaterials provide enough space for bone
growth. Polypropylene Carbonate (PPC) is a high molecular with higher elastic it and
lower glass transition temperature (Tg). In previous study, PPC have shown
biodegradable characteristic. However, PPC is not a natural polymer, resulting in poor
biocompatibility. Therefore, producing high biocompatibility befalls technical
difficulties. The purpose of this study was to enhance the biocompatibility of the PPC
using composite material technology and sault-leaching technique; PPC was mixed
with poly-lactic acid (PLA) and tricalcium phosphate (TCP) to produce the
PPC/PLA/TCP composite scaffold (PPTCS). After combining with 2% PLA and 8%
TCP, the water contact angle was decreased compared with PPC. On PPTCS, the
sault-leached composite possessed the pore size about 111.12 μm and the porosity
was even in PPTCS. The in vitro model was shown that MC3T3-E1 cells can adhere
on PPTCS with time-dependently increases in cell proliferation, indicating that
PPTCS is well biocompatible. After mouse bone defect was filled with PPTCS, the
trabecular bone number and trabecular bone connectivity density increased, compared
to bone defect without hilling with PPTCS. The histologic analyses shown that there
was no damage in liver tissue. These results have demonstrated that PPTCS is a
porous and biocompatible material, which is non-toxicity and can promote bone
healing.

並列關鍵字

Bone healing ； Biomaterials ； Composite Scaffold

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