小葉葡萄改善高血糖之活性研究

Study of Vitis thunbergii var. taiwaniana on the improvement of hyperglycemia in streptozotocin-induced diabetic mice

李文彬(Wen-Pin Li)

指導教授 ： 梁有志

臺北醫學大學/醫學科技學院/醫學檢驗暨生物技術學系/碩士(2012年)

https://doi.org/10.6831/TMU.2012.00010

摘要

目前全世界有超過 1.71 億糖尿病患者，預計到 2030 年，這一數字將高達3.66 億。台灣在 2010 年，糖尿病患者約有一百萬至一百二十萬人，平均每 1小時 4 分鐘就有 1 人死於糖尿病，死亡率為 22 人/日，排序十大死因的第五位。台灣糖尿病的死亡人數也逐年增加中，逐漸攀高的糖尿病盛行率及其防治與治療已被視為非常重要的課題。因此，激勵許多科學家開發新的藥物與功能性食品來改善糖尿病。
小葉葡萄 (Vitis thunbergii var. taiwaniana ) 中富含白藜蘆醇 (resveratrol)、槲皮素 (quercetin) 及其他多酚類化合物，這些多酚類化合物在很多的動物實驗中，被證實能改善高血糖。本研究的目的是評估小葉葡萄在鏈佐菌素 (streptozotocin, STZ) 所誘導的第二型非胰島素依賴型糖尿病 (NIDDM) 鼠體內其改善糖尿病的性質。首先，小葉葡萄的葉、根及莖的酒精萃取物，被用來研究 STZ 誘導糖尿病小鼠之降血糖效果。然而，這 3 種酒精萃取物皆没有任何抑制血糖的作用。第二，小葉葡萄的葉及莖的熱水萃取物，分别給予 STZ 誘導之糖尿病小鼠 4 週。有趣的是，只有小葉葡萄的葉熱水萃取物有顯著的降低血糖水平，但是莖的萃取物並無此效果。第三，對 STZ 誘導之糖尿病小鼠，餵食小葉葡萄的熱水萃取物 30 分鐘後，進行血糖測試，結果發現餵食小葉葡萄葉熱水萃取物後的 2 及 3 小時，其高血糖有明顯的降低。另外，從 STZ 誘導的糖尿病小鼠胰臟組織切片發現，小葉葡萄的葉熱水萃取物具有保護胰臟 β 細胞的功能。從這些結果顯示，小葉葡萄的葉熱水萃取物具有降低高血糖的活性，可以作為保健食品的開發，來防止糖尿病。

關鍵字

小葉葡萄 ； 鏈佐菌素 ； 糖尿病 ； 高血糖

並列摘要

At present, there are more than 171 million people with diabetes around the world. It’s expected the number will reach 366 million by 2030. In 2010, Taiwan have about 1 million to 1.2 million diabetic patients that is one death every one hour and 4 minutes on average, and the mortality is 22 people per day. Diabetes is the 5th leading cause of death among Taiwan, and the mortality is also increasing. The rising prevalence as well as the prevention and therapy of diabetes have been regarded as very important issues. Therefore, many scientists are urging to develop novel medicine or functional food to improve the diabetes.
Vitis thunbergii var. taiwaniana (VTT) is rich in resveratrol, quercetin, and other polyphenols. Those compounds have been known to improve the hyperglycemia in several experimental models. The aim of this study was to evaluate the antidiabetic properties of VTT in streptozotocin (STZ)-induced non-insulin-dependent diabetes mellitus (NIDDM) mice. First, the alcohol extracts of VTT leaf, root, and stem were used to examine the hypoglycemia effects in STZ-induced DM mice. However, three kinds of alcohol extracts did not have any inhibitory effects in blood glucose level. Second, the hot water extracts of VTT leaf and stem were given to the STZ-induced DM mice for 4 weeks. Interestingly, only leaf extracts significantly decreased blood glucose level but the stem extracts did not have any effect in blood glucose level. Third, the STZ-induced DM mice were begin to examine the glucose level after feeding with hot water extracts for 30 minutes. The hyperglycemia were obviously reduce between 2 and 3 hours after feeding the leaf hot water extracts of VTT. In addition, pancreatic tissue sections from STZ-induced diabetic mice found that leaf hot water extracts of VTT have the protection of pancreatic β cell function. These results suggest that leaf hot water extracts of VTT exhibited hypoglycemia activity, and might be developed as functional food to prevent diabetes mellitus.

並列關鍵字

Vitis thunbergii var. taiwaniana ； streptozotocin ； diabetic mellitus ； hyperglycemia

參考文獻

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