補陽生藥萃取物之體外、體內抗氧化活性研究

In Vitro and In Vivo Antioxidative Study of Epimedium Extract

石偉志(Wei-Chih Shih)

指導教授 ： 何意

臺北醫學大學/藥學院/藥學研究所/碩士(2012年)

摘要

糖尿病為一慢性疾病，隨著人類生活以及醫療水準的提高，糖尿病的罹患率並未因此而降低。若能延緩胰島細胞功能的喪失，對於糖尿病患者的血糖及病程的控制皆有正面意義。本實驗利用淫羊藿生藥粗萃取物(EMF)以不同濃度對C57BL/6小鼠及RINm5F cell以〔1,1-Diphenyl-2-picrylhydrazyl (DPPH) assay、Oxygen radical absorbance capacity (ORAC) assay及Malodialdehyde (MDA)及、Glutathion (GSH)含量測定〕，探討淫羊藿粗萃物是否有助益於RINm5F cell的保存及小鼠體內的抗氧化能力的提升。
研究結果顯示，以淫羊藿粗萃物進行DPPH assay，在100μg/ml時的自由基清除能力約為15.6μM Ascobic acid的1.3倍；10μg/ml的淫羊藿粗萃物於ORAC assay的總抗氧化能力約與trolox 25uM 及catechin 1ug/ml相當。以H2O2誘導胰島細胞產生氧化壓力後，淫羊藿粗萃物對於產生氧化壓力的胰島細胞有顯著的抗氧化力提升 (p<0.05)。
以淫羊藿萃取物固定劑量多次重複給藥，在乙醇引發氧化壓力的壓力組小鼠，其體內的總抗氧化力有顯著的下降 (P<0.05)，合併給予乙醇以及淫羊藿粗萃物組，其總抗氧化力也有上升的趨勢。以固定劑量，一天投予兩次25mg/kg淫羊藿萃取物，連續投予八天後，MDA有顯著的下降 (P<0.05)，其結果說明長時間投予淫羊藿萃取物對於預防脂質過氧化作用，是有意義的。

關鍵字

補陽藥 ； 生藥 ； 體外體內研究 ； 抗氧化

並列摘要

Abstract
Diabetes mellitus is a chronic non-communicable disease. Although the high quality of life and advance of medicine has been achevied, it still did not drop down in the prevalence of diabetes. Preserving islet β-cell function has the benefit to control blood sugar and to prevent the progressing deterioration caused by diabetes mellitus.
In this thesis, the effect of Epimedium sagittatum Maxim extract (EMF)at different concentrations on C57BL/6 mice and RINm5F cell line have been studied with DPPH、ORAC assay and plasma concentration of MDA、GSH are also determinated to interpret the promopting antioxidative capacity of EMF in vivo.
The results of our research showed that: the radical scavenges capacity of epimedium crude extract (100μg/ml) is 1.3 times higher than that of Ascorbic acid (15.6 μM) measured by DPPH assay. The oxygen radical absorbance capacity (ORAC) of epimedium crude extract (10 μg/ml) is equivalent to those of trolox (25uM) and catechin (1ug/ml). Epimedium crude extracts show the trend of increasing in antioxidation (P<0.05) after H2O2 induced oxidatative stresses in RINm5F cell.
After multiple dose (10, 25 mg/kg) of EMF administration to mouse, the oxygen radical absorbance capacity has been significantly decreased in alcohol induced stress mouse group (P<0.05). It also showed the trend of increase in oxygen radical absorbance capacity in both alcohol induced stress and EMF administration group. There was a noticeable decrease (P<0.05) in the plasma concentration of malodialdehyde (MDA) after multiple doses that were administrated twice daily for a period of eight days, the results showed that it is effective to prevent the lipid peroxidation after a long-term with administration of Epimedium crude extracts.

並列關鍵字

RINm5F cell ； ORAC assay ； Epimedium ； In Vitro In Vivo study ； Antioxidation

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