- 學位論文
Study of Phytochemistry and Bioactivity of Constituents from Fruits of Aquilaria crassna
Study of Phytochemistry and Bioactivity of Constituents from Fruits of Aquilaria crassna
並列摘要
The resinous portions of Aquilaria plants, called Aquilaria crassna, have been used as medicines and incenses. It contains a great variety of sesquiterpenes. This oil is widely used among various countries as perfumes and ingredient in medical recipes. The present study aims to analyze the phytochemistry of medicinal plant, Aquilaria crassna fruit. The specific objectives of the study are to extract A.crassna by using 100% methanol and to isolate the compounds using open column, and medium pressure liquid chromatography (MPLC) methods. In addition to this, the study has also been conducted to elucidate the structures of isolated pure compounds using nuclear magnetic resonance spectroscopic techniques. The research was designed with an experimental method. The dried A.crassna (6.13 kg) was extracted three times by 100% MeOH, at room temperature after 3 days. Pure compounds were isolated from MeOH extract using various column chromatographical techniques. The elucidated pure compounds from ethyl acetate layer were identified to be iriflophenone 2-O-α-rhamnopyranoside (1),4-hydroxybenzoic acid (2), mangiferin (3), rhamnocitrin-3-O-β-D-glucoside (4) kaempferol-3-(6′-coumaroyl) glucoside (5). The structures of these compounds were determined by extensive spectroscopic analyses, including 1D and 2D NMR.