應用於微粒分離之電紡奈米孔洞之聚乳酸微管陣列薄膜

FABRICATION OF ELECTROSPUN NANO-POROUS MICROTUBE ARRAY MEMBRANE (MTAM) FOR PARTICLE SEPARATION

趙子豪(Chew Chee Ho)

指導教授 ： 陳建中

共同指導教授 ： 吳其昌

臺北醫學大學/醫學工程學院/生醫材料暨組織工程研究所/碩士(2016年)

摘要

在傳統微過濾中，使用的陶瓷材料及高分子聚合物薄膜，常被改良並應用於奈米過濾，但這些薄膜具有孔隙率較低及滲透性差等特性。隨著“奈米時代”的到來，對於品質較好的奈米過濾薄膜，其需求已有顯著增加。近來，一種被稱為新穎微管陣列薄膜（MTAMs）的靜電紡絲中空纖維薄膜已被開發出來並且應用在奈米過濾其相關領域。本研究中兩種類型之靜電紡絲新穎微管陣列薄膜（超薄型PLLA MTAMs和正常厚度之PLLA MTAMs）在混合不同分子量之致孔劑後（分子量：4600，8000和20000 kDa），並在6千到8千伏特不等之電壓，及濕度55±4％和溫度23±3℃的條件下所製成。微觀結構分析中顯示其薄膜孔徑分別為0.03±0.02微米到0.09±0.02微米之間（超薄型PLLA MTAMs）和 0.02±0.004微米到0.11±0.01微米之間（正常厚度之PLLA MTAMs）。其微管之管壁的厚度分別在2.70±0.53微米到2.24±0.33微米的範圍內（超薄型PLLA MTAMs）；和5.874±1.63微米到5.16±1.58微米（正常厚度之PLLA MTAMs）。在孔隙率測試中顯示，PLLA / 20 PEG 分子量20000薄膜的孔徑分佈從299奈米到60奈米（12.01psi），PLLA / 20 PEG分子量4600薄膜的孔徑分佈則是230奈米到77奈米（10.07 psi）。超薄型PLLA MTAMs與正常厚度之PLLA MTAMs相較下，超薄型PLLA MTAMs有較顯著的強度，其最大拉伸強度為5.48±0.32MPa及楊氏係數869.60±55.16MPa。 經紫外光光譜儀測試後與Sigma公司所提供的數據分析對照後得知PLLA / 20 PEG分子量 4600、8000和20000的超薄型PLLA MTAMs其微粒過濾之尺寸分別為：<5奈米，30奈米和60奈米，然而正常厚度之PLLA MTAMs微粒過濾之尺寸則分別為：沒有檢測到微粒，40奈米和80奈米。將PLLA / 20 PEG 分子量4600＆8000之薄膜結合過濾系統後可成功分離出30奈米大小的微顆粒。結果顯示，正常厚度之PLLA MTAMs薄膜可成功過濾並分離奈米金微粒並將其使用在藥物載體應用當中。

關鍵字

聚乳酸（PLLA） ； 微管陣列薄膜（MTAM） ； 靜電紡絲 ； 奈米金微粒 ； 奈米過濾

並列摘要

Traditionally, ceramic/polymeric membranes used in micro-filtration were adapted for nano-filtration. These membranes were mechanically poor with low permeability and porosity. With the arrival of the ‘nano-age’, demand for superior nanofiltration membranes increased significantly. Recently, a novel class of electrospun hollow fibers known as Microtube Array Membranes (MTAMs) was developed and adapted for nanofiltration application. Two types of electrospun MTAMs (ultra-thin and normal thickness) with different molecular weight of porogenic compound (Mw: 4600, 8000 and 20000 kDa) were fabricated at a voltage range of 6-8 kV; relative humidity of 55±4% and a temperature of 23±3°C. Microstructure analysis revealed the pore sizes were between 0.03±0.02 µm-0.09±0.02 µm (ultra-thin PLLA MTAMs) and 0.02±0.004 µm-0.11±0.01 µm (normal thickness PLLA MTAMs). Lumen thickness were in the range of 2.70±0.53 µm-2.24±0.33 µm (ultra-thin MTAMs); and 5.874±1.63 µm-5.16±1.58 µm (normal thickness MTAMs). Porometry testing revealed that PLLA/20 PEG 20000 registered a pore size distribution from 299 nm-60 nm (12.01 psi) and 230 nm-77 nm (10.07 psi) for the PLLA/20 PEG 4600. Ultra-thin MTAMs were significantly stronger than normal thickness MTAM with a maximum tensile strength of 5.48±0.32 MPa and a Young’s modulus of 869.60±55.16 MPa. UV spectrophotometer analysis with comparison data obtained from Sigma of the filtrate particle size of PLLA/20 PEG 4600; 8000 & 20000 were <5 nm, 30 nm and 60 nm (ultra-thin MTAM); and no particles detected, 40 nm and 80 nm (normal thickness MTAM). Combined filtration of PLLA/20 PEG 4600 & 8000 produced particles size of 30 nm. The results indicated that potentially, normal thickness PLLA/20 MTAMs can be used in gold nano-filtration applications for drug conjugate use.

並列關鍵字

Polylactic Acid (PLLA) ； Microtube Array membrane (MTAM) ； Electrospinning ； Gold Nano-particle ； Nano-filtration

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