初生期注射丙酸睪丸脂酮影響前青春期大白鼠卵泡生長之形態計量研究

Morphometric Studies of Neonatal Treatment with Testosterone

許政成(Hsu Chen Cheng)

指導教授 ： 陳慶源

臺北醫學大學/醫學院/天然物醫學研究所/碩士(1994年)

摘要

無資料

關鍵字

丙酸睪丸脂酮 ； 卵泡 ； 前青春期 ； 大白鼠

並列摘要

Wistar品系之雌性大白鼠, 於出生後第2天開始, 每天施予皮下(S.C.)注 射 0.02ml 劑量 , 含有 250μg/ml ( 溶於芝麻油中 ) 的丙酸睪丸酯酮 (testosterone propionate ; 簡稱 T.P.),連續10天共計 50μg 丙酸睪 丸脂酮之注射劑量。對照組僅注射以芝麻油溶劑,注射時間、劑量及天數 如同實驗組之處理方式。實驗組與對造組的雌性大白鼠, 於出生後每滿 14、21、21、28、35 和 56天時取出右側卵巢, 經由慣用的組織切片製作 程序作連續切片, 厚度為5μg, 以便觀察卵泡的發育狀態，在前青春期 (prepu- bertal stage) 的 14、21、28天時，T.P.處理組早期的初級卵 泡 (early stage of primary follicles)---TypeⅡ (具單層立方形的濾 泡細胞) 和 Type Ⅲ (具單層柱狀的濾泡細胞) 卵泡的數目總和以及 Type Ⅶ(具 fo- llicular antrum) 卵泡的數目比對照組有顯著地增加。 意即出生後第2天開始，每天注射 5μg T.P.連續10天，總劑量為50μg於 前青春期的 14、 21、28天時，發現可促進卵泡的早期生長，並誘導在卵 巢中積蓄較多的 antral follicles Female albino rats of an inbred Wistar strain had been daily given a subcutaneous injections either 0.02 ml vehicle only, or 5 μg testosterone propionate (T.P.) in the same volume of sesame oil on the next day after birth, and then continued this process for 10 days long. Total dose of T.P.received by each T. P.-treated group was 50 μg. All right ovaries were removed at 14、21、28、 35、56 days after birth. Growth of ovarian follicles was examined with their serial sections made by the routine histological tech- niques. In T.P.-treated groups, either the sum of numbers of TypeⅡ follicles (growing oocyte surrounded by single layer of cuboid -shaped follicular cells) and Type Ⅲ follicles (growing oocyte surrounding by single layer of column-shaped follicular cells) or the sum of numbers of Type Ⅶ follicles (antral follicles) were more than those of the control(sesame oil-treated) groups at pre- pubertal stage (e.g.,14、21 or 28 days after birth). From these points of views, the author finds meaningfully in any prepubertal group of T.P.-treated rats, the total dose of T.P. 50 μg, given neonatally for 10-day period, has been proved to stimulate signi- ficantly the growth of the early stage of primary follicles and preserves more numbers of the antral follicles in ovaries of the prepubertal female rats.

並列關鍵字

testosterone propionate ； ovarian follicles ； prepubertal rats