躁症患者免疫功能之研究

Immunological Functional Study in Patients with Mania

周月梅(Yueh-Mei Chou)

指導教授 ： 呂思潔

臺北醫學大學/醫學院/細胞及分子生物研究所/碩士(2000年)

摘要

有許多文獻證據顯示中樞神經系統與免疫系統間有密切的交互作用，比較特別的是有關精神壓力與精神疾病患者有免疫異常情形的描述。目前學者提出精神裂症患者有單核細胞與T細胞活化的假說，也有學者提出自體免疫機制可能是精神分裂症的發病因素之一。躁鬱症是一種可能會出現躁狀精神狀態的情感性疾病，目前有關躁症的免疫研究較少且結果仍有爭議，因此本實驗的目的主要是研究單核細胞與T細胞所分泌的細胞激素的表現情形，以及躁症患者可能存在的自體免疫抗體。T細胞與單核細胞的數目在躁症患者與正常控制組相比沒有改變，躁症患者血清中sIL-2R的濃度有意義的高於正常控制組，由PHA所刺激產生之IFN-g的濃度在躁症患者急性期與緩解期皆明顯有意義的低於控制組，由LPS所刺激的單核細胞激素中只有LIF有意義的高於正常組。總而言之，這些結果顯示雙極性情感疾病的免疫病生理機制可能受症狀嚴重程度的影響，且調節機轉與正常人不同。另外，本實驗還利用西方點墨法來檢測躁症患者血清樣本中與U-373 MG神經母細胞瘤細胞株蛋白萃取物結合的抗體。有19.6﹪的躁症患者與10.6﹪的正常控制組表現對抗60 kDa蛋白的抗體，還有7.2%的患者與15.2%的正常人會表現對抗100-120 kDa蛋白的抗體，而上述這些抗體為何則需再進一步確認。

關鍵字

躁症 ； sIL-2R ； IFN-g ； 自體抗體

並列摘要

There is now a large body of evidence suggesting a close interaction between the central nervous system and the immune system. More specifically, immunological abnormalities have been described in relation to psychological stress and in patients with psychiatric illness. Recently, it has been hypothesized that monocytes and T lymphocytes are activated in patients with schizophrenia. Furthermore, an autoimmune mechanism was also suggested as a possible factor in the pathogenesis of schizophrenia. Bipolar disorder is a mood disorder that may manifest psychosis in manic episode. Reports on the assessment of immunity in mania are limited and remain controversial. The aims of this study were to investigate the monocytic and T lymphocyte dysfunction hypothesis and the possible autoantibodies involved in patients with mania. The number of T cells and monocytes remain unchanged in the manic patients versus normal control. The soluble IL-2 receptor (sIL-2R) is significantly higher in mania than normal volunteers. PHA-induced IFN-g is significantly decreased in acute mania and remission stage as compared to controls. LPS-induced LIF is significantly higher than controls. Taken together, these results shown a state-dependent effect on immune pathophysiology of bipolar disorder probably exits and the modulatory mechanism may vary from normal. The presence of antibodies against brain antigens protein extract from U-373 MG glioblastoma cell line was investigated in the serum of patients with mania. There were 19.6% of patients with mania and 10.6% of normal subject presence of antibodies against protein 60 kDa. The antibodies against 100-120 kDa was present in 7.2% of patients with mania and 15.2% of normal subjects. The presence of antibodies against these proteins should be investigated as possible specific indicators.

並列關鍵字

mania ； sIL-2R ； IFN-g ； autoantibodies

參考文獻

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