中藥材之炮製---地 黃

The Processing of Chinese Medicinal Material Rehmanniae Radix

李榮國(Rong-Gwo Lee)

指導教授 ： 楊玲玲

臺北醫學大學/藥學院/生藥學研究所/碩士(2000年)

摘要

近年來各研究界自地黃及其他不同植物中分離出新的Iriroid Glycosides且大多為catalpol 衍生物，此類Iriroid化合物之生理活性研究文獻報告頗多，故本研究化學方法選擇地黃中屬Iriroid Glycosides之catalpol為指標成分，經參考傳統地黃之炮製「九蒸九曬」及現代炮製之一般修治、水火共製等方法，並改良以現代科學方法，利用高壓蒸製法進行探討，以清蒸及酒蒸法為主，並另以傳統常壓蒸製進行對照，原材料除整粒進行炮製外，比較各種炮製方法之差異，另包括切片及不同炮製次數。 本研究利用高敏感度之高效液相層析法(HPLC)定量各種炮製方法飲片中catalpol 成分之變化，並加以比較。同時亦收集市售地黃炮製飲片，以進行市售品catalpol之含量調查，本實驗建立catalpol之分析方法，檢測各種炮製品結果以清蒸高壓及酒製高壓兩種蒸製方式，可獲得catalpol含量較高之熟地黃，根據本實驗測定台灣市場市售熟地黃飲片其catalpol含量較高壓炮製地黃低，亦比市售生地黃飲片為低。而市售生、熟地黃catalpol含量測定，與文獻所記載者相符，即以一般修治及水火共製等方法炮製之地黃中Iriroid Glycosides之catalpol、6〝-o-acetylcatalpol、aucubin等均有明顯減少或消失，而以加壓方式所得到之catalpol含量明顯增加。

關鍵字

地黃 ； 酒蒸清蒸 ； Catalpol ； 常壓 ； 高壓 ； 炮製 ； 市售品 ； HPLC定量

並列摘要

Some novel Iriroid glycosides were isolated recently from Rehmannia glutinosa Libosch var. hueichingensis, and several previous papers reported that Iriroid related compounds performed many biological and physiological activities including antioxidative and anti-inflammatory activities. In the present study, we focused on the study the different procession techniques of Rhemanniae Radix on the production of catalpol, an Iriroid Glycosides in Rehmannia glutinosa Libosch var. hueichingensis, by high performance liquid chromatography (HPLC). The results demonstrated that cooked Rhemanniae Radix prepared by traditional procession techniques including steam with high pressure and alcholization produced higher content of catapol. Investigation of the catapol content of cooked Rhemanniae Radix in Taiwan markets showed that marketed cooked Rhemanniae Radix chips contained lower amount of catalpol than those made by high pressure. The results of the present study provided a technique to analyze the amount of catapol in Chinese medicinal product Rhemanniae Radix as a guide for raw material preparation in manufacturing the high quality and stable Chinese medicinal products.

並列關鍵字

Dry and cooked form ； Rehmanniae Radix ； processing ； Catapol ； HPLC analysis

參考文獻

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