躁症患者抗熱休克蛋白-60、-70及-90自體抗體之分析

Analysis of Anti-Heat Shock Protein 60, -70 and -90 Autoantibodies in Patients with Manic Episode

林佳儀(Chia-Yi Lin)

指導教授 ： 呂思潔

臺北醫學大學/醫學院/細胞及分子生物研究所/碩士(2002年)

摘要

中樞神經系統與免疫系統間有密切的交互作用，在許多文獻中多獲得證實，其中有關精神壓力與精神疾病患者免疫異常情形的研究，引起我們的重視。比較特別的是，自體免疫機制被證實是精神分裂症可能的發病原因之一。本實驗的目的主要是檢測躁症患者對於抗熱休克蛋白抗體的表現。本實驗的血液樣本是取自躁期病患。患者的血清以西方點墨法檢測與U-373MG神經母細胞瘤細胞株細胞萃取物結合的抗體的存在，並進一步以ELISA測定抗熱休克蛋白的濃度。熱休克蛋白（Hsps）存在各種細胞中，且具有對抗感染及壓力的保護功能。Hsps於組織中異常的表現並與免疫系統發生交互作用，可能導致自體免疫疾病，例如兒童糖尿病或類風濕性關節炎。 在抗Hsp90自體抗體部分，我們以ELISA檢測的結果發現，未用藥急性期患者抗Hsp90抗體比緩解期有意義的增加。已用藥急性期抗Hsp90抗體比未用藥急性期患者有意義的降低。已用藥急性期抗Hsp70及抗Hsp90自體抗體比正常控制組有意義的降低。而血清中抗Hsp60自體抗體在患者及正常控制組間沒有顯著意義的差別。我們推測急性躁期患者在Hsp90有免疫異常的反應, 這種現象可能與躁症急性期的發病有關聯。

關鍵字

躁症 ； 自體免疫 ； 自體抗體 ； 熱休克蛋白

並列摘要

A close interaction between the central nervous system and the immune system have evidenced in many documents, in which we have paid attention to the study of immunological abnormalities both in psychological stress and patients with psychotic disorder. More specifically, an autoimmune mechanism was suggested as one of the possible factors in the pathogenesis of schizophrenia. The aim of this study was to investigate the presence of antibodies that react with heat shock proteins (Hsps) in sera from patient with mania. The sera were tested for antibodies binding to cell extracts of U-373MG glioblastoma cell line using western blot analysis. Hsps are intracellular proteins in most cells which are thought to serve protective functions against infection and cellular stress. In some instances, Hsps may be aberrantly expressed on target tissues interaction with the immune system might potentially result in autoimmune disease, as suggested in juvenile diabetes mellitus or rheumatoid arthritis. In the part of autoantibodies against Hsp90, we observed that un-medicated acute patients were significantly higher compared with remission patients. Sera anti-Hsp90 autoantibodies in medicated acute patients were significantly lower compared with un-medication acute patients. Sera anti-Hsp 70 and 90 autoantibodies in medicated acute patients were significantly lower compared with normal controls. In addition, there was no significant difference on autoantibodies against Hsp60 between patients and normal controls . We suggested that a presence of abnormal immune reactivity involving Hsp90 in patients with manic acute episode, and it may implicate in pathogenesis in acute episode with this disease.

並列關鍵字

mania ； autoimmunity ； autoantibody ； heat shock protein

參考文獻

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