白鳳菜酚性成份及其自由基清除活性研究

PHENOLIC CONSTITUENTS AND FREE RADICAL SCAVENGING ACTIVITIES OF GYNURA FORMOSANA

黃盈華(Ying-Hua Huang)

指導教授 ： 李美賢 ； 侯文琪

臺北醫學大學/藥學院/生藥學研究所/碩士(2002年)

摘要

中文摘要 白鳳菜(Gynura formosana Kitamura)為臺灣固有種菊科(compositae) 植物，民間常用做蔬菜，亦被用來治療高血壓、糖尿病及腦中風等。本研究以白鳳菜之70% 丙酮萃取物，經FeCl3，DPPH自由基清除活性及還原力測定，進行預試驗，發現白鳳菜具有成為天然食用可清除自由基的潛力。故乃大量採集以70%丙酮萃取，配合酚性成分及活性追蹤進行化學成分分離，經由Diaion HP20、Toyopearl HW 40(C)、MCI CHP 20P、Sephadex LH20、ODS (Octadecylsilane) 等管柱層析，及高效能液相層析法 (high performance liguid chromatography, HPLC) 得到四個化合物，利用化學反應及光譜分析進行結構鑑定，化合物1為quercetin 3-O-rutinoside (rutin) (1)，化合物2為caffeic acid (2)，化合物3為kaempferol 3-O-rutinoside (nicotoflorin) (3)，化合物4為kaempferol 3-O-robinobioside (4)。其中quercetin 3-O-rutinoside (rutin) (1)對DPPH (1,1-diphenyl- 2-picryl- hydrazy)自由基清除能力之IC50為2.3 μg/mL，且於還原力測定中，效果最佳。Quercetin 3-O-rutinoside (rutin) (1), kaempferol 3-O-rutinoside (nicotoflorin) (3), kaempferol 3-O-robinobioside (4) 利用電磁自旋共振儀 (EPR) 偵測DPPH自由基試驗，發現具有濃度依存性之抑制作用。本研究由白鳳菜中分離得到酚性成分且與清除自由基之活性有關，未來白鳳菜應可更為推廣栽培，且可朝健康食品之方向發展。

關鍵字

白鳳菜 ； 酚性成分 ； DPPH自由基清除活性 ； 還原力測定

並列摘要

Abstract Gynura formosana Kiamnra (Compositae) is a folkic herbal medicine in Taiwan. G. formosana was popular used as vegetable and to treat the hypertension, diabetes mellitus, cerebral infarction etc. In the screening test with FeCl3 T.S. reagent, scavenging activity of DPPH activity, and reducing power, it implied G. formosana is the potential condidate developing to the natural health food because of its scavenging activities. Thus, the herb of G. formosana was extract with 70% aqueous acetone, then isolated chemical components by using both FeCl3 T.S. and bio-guide. The 70% aqueous acetone extract was chromatographed over Diaion HP20, Toyopearl HW40(C), MCI CHP 20P, Sephadex LH20, ODS (Octadecylsilane) columns, and purified by HPLC (high performance liguid chromatography) to yield four compounds, compound 1 to 4. The structures were identified by chemical reactions and spectroscopic methods. Compound 1 to 4 are characterized as quercetin 3-O-rutinoside (rutin) (1), caffeic acid (2), kaempferol 3-O-rutinoside (nicotoflorin) (3), and kaempferol 3-O-robinobioside (4), respectively. The IC50 value of quercetin 3-O-rutinoside (rutin) (1) was 2.3 μg/mL on DPPH (1,1-diphenyl-2- picrylhydrazy) free radical scavenging activity. And reducing power of (quercetin 3-O-rutinoside) rutin (1) was also the showed the best activity. In the EPR analysis, rutin (1), kaempferol 3-O-rutinoside (nicotoflorin) (3), and kaempferol 3-O-robinobioside(4) were showed the scavenging activities of DPPH free radicals in a dose-dependent manner. In this study, the four phenolic components were isolated from G. formosana and exhibit the free radicals activity. Therefore, G. formosana may be develop to the health food in the future.

並列關鍵字

EPR ； rutin ； caffeic acid ； nicotoflorin ； kaempferol 3-O-robinobioside

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