- 學位論文
共生質對大白鼠腸道功能與體內抗氧化狀態之影響
Effects of synbiotics on the intestinal function and antioxidative status in rats
摘要
本研究主要探討共生質對於大白鼠腸道功能與體內抗氧化狀態之影響。將雄性Sprague-Dawley大白鼠分成對照組、低劑量組和高劑量組(添加1.56及7.80g/kg/day共生質)等三組。共生質含有菊糖及五種益菌 (常寶寧；美商威望股份有限公司)。實驗第八週進行糞便中菌相之分析後，進行空腸黏膜脂解?和雙醣?(lactase、isomaltase、sucrase)活性測定，並進行空腸黏膜、肝臟與紅血球抗氧化酵素活性及抗氧化物質(GSH)之分析。結果發現，低劑量組糞便中大腸桿菌菌數顯著降低，空腸黏膜lactase活性則顯著提高，而且紅血球GRd活性也顯著增加。而高劑量組大白鼠除糞便中大腸桿菌菌數顯著降低以外，糞便中乳酸桿菌及雙叉桿菌菌數也顯著增加，並且發現空腸黏膜脂解?、lactase、isomaltase活性顯著提高，而且發現空腸黏膜glutathione reductase(GRd)、catalase (CAT)及superoxide dismutase (SOD)活性顯著增加，GSH的濃度也顯著提升，另外，肝臟中CAT活性與紅血球GRd與SOD活性也顯著增加。由此可知，大白鼠攝取共生質粉末不僅可以改善腸道菌相平衡、增加空腸黏膜消化酵素活性，更可有效提升體內抗氧化狀態。
關鍵字
共生質，腸道功能，體內抗氧化狀態並列摘要
This study was to investigate the effects of synbiotics on the intestinal function and antioxidative status in rats. The male Sprague-Dawley rats were randomly divided into three groups such as control (C), low (L) and high dosage (H) groups (1.56 and 7.80g/kg BW/day synbiotics powder was added to diet). The synbiotics was composed of inulin and 5 probiotics (FloraGuard?, Viva Life Science). After 8 weeks, rats in L group showed the significantly lower fecal E. coli count than that of control group. The jejunal lactase activity and erythrocytic GRd activity were significantly increased in L group. Rats in H group had higher fecal Lactobacillus and Bifidobacterium and lower E. coli. counts than those of C group. H group also showed the significant increase in jejunal lipase, lactase and isomaltase activities. In addition, the jejunal GRd, CAT and SOD activities, erythrocytic GRd and SOD activities, and hepatic CAT activity were significantly increased in H group.