植物多醣萃取物對肝癌與T淋巴癌細胞增殖之影響

本研究探討熱水萃取之枸杞多醣萃取物（LBPE）及地黃多醣萃取物（RPGE）對老鼠肝癌細胞株（H-4-II-E）、人類肝癌細胞株（HA 22T/VGH）及小鼠T淋巴癌細胞株（EL4. IL-2）細胞增殖之影響。添加不同劑量之LBPE（1、2、5 mg/mL）與RGPE（1、2、5、10 mg/mL）與H-4-II-E共同培養6~24小時後，結果顯示LBPE 2、5 mg/mL及RGPE 5、10 mg/mL可劑量效應抑制細胞增殖；同時，給予LBPE 5 mg/mL及RGPE 5、10 mg/mL培養6~12小時即造成H-4-II-E細胞程式化凋亡；LBPE 2、5 mg/mL及RGPE 5、10 mg/mL培養18~24小時後凋亡細胞產生越顯著，並且在24小時後劑量效應的誘導H-4-II-E細胞p53蛋白質表現，其表現量分別為控制組的118.7%、143.0%、110.1%及131.6%。對於HA 22T/VGH細胞增殖而言，添加LBPE 2、5、10 mg/mL或RGPE 2、5、10 mg/mL培養24小時後具有劑量抑制效應；LBPE 10 mg/mL可產生細胞凋亡。此外，LBPE（1、2、5 mg/mL）與RGPE（1、2、5、10 mg/mL）單獨或伴隨phorbol 12-myristate 13-acetate（PMA）的刺激，在共同培養24小時後，可劑量效應抑制EL4. IL-2細胞增殖，並且單獨添加LBPE（2、5 mg/mL）或RGPE（5、10 mg/mL），可降低其白介質素-2（interleukin 2 ; IL-2）的分泌量，同時也可以改善因PMA所刺激IL-2的分泌情形。

關鍵字

肝癌；枸杞多醣萃取物；地黃多醣萃取物； p53蛋白質；凋亡細胞

並列摘要

The study investigated the effects of hot water-extracted Lycium barbarum polysaccharides (LBPE) and Rehmannia glutinosa polysaccharides (RGPE) on cell proliferation in rat hepatocellular carcinoma (H-4-II-E), human hepatocellular carcinoma (HA 22T/VGH) and mouse T lymphoma (EL4. IL-2) cells. Cells were incubated with LBPE (1, 2, 5, 10 mg/mL) or RGPE (1, 2, 5, 10 mg/mL). After 6~24-h treatment, LBPE (2, 5 mg/mL) and RGPE (5, 10 mg/mL) dose-dependently inhibited proliferation in H-4-II-E cells determined by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phen- yl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. The apoptotic cells appeared in H-4-II-E cells after 6- and 12-h treatment with LBPE (5 mg/mL) or RGPE (5, 10 mg/mL) and after 18- and 24-h treatment with LBPE (2, 5 mg/mL) or RGPE (5, 10 mg/mL) determined by flow cytometry using propidium iodide (PI) stain. The expression of p53 proteins in H-4-II-E cells treated with LBPE 2, 5 mg/mL and RGPE 5, 10 mg/mL for 24 h was 118.7%, 143.0%, 110.1% and 131.6%, respectively. Additionally, LBPE (2, 5, 10 mg/mL) and RGPE (2, 5, 10 mg/mL) dose-dependently inhibited proliferation in HA 22T/VGH cells after 24 h.The apoptotic cells appeared by the treatment of 10 mg/mL LBPE. In EL4. IL-2 cells, LBPE (1, 2, 5 mg/mL) and RGPE (1, 2, 5, 10 mg/mL) dose-dependently inhibited proliferation after 24-h treatment with or without phorbol 12-myristate 13-acetate (PMA) stimulation. The secretion of interleukin-2 (IL-2) with or without PMA stimulation was suppressed by LBPE (2, 5 mg/mL) and RGPE (5, 10 mg/mL) after 24-h treatment in EL4. IL-2 cells. Therefore, LBPE and RGPE significantly affected H-4-II-E and HA 22T/VGH cell proliferation in time- and dose-dependant manners, and drove cell death by apoptosis and p53 protein expression at higher doses in H-4-II-E cells. Additionally, LBPE and RGPE suppressed PMA-stimulated cell growth and IL-2 secretion in EL4. IL-2 cells.

並列關鍵字

hepatocellular carcinoma ； Lycium barbarum polysaccharides (LBPE) ； Rehmannia glutinosa polysaccharides (RGPE) ； p53 protein ； apoptotic cells

參考文獻

Almog N, Li R, Peled A, Schwartz D, Wolkowicz R, Goldfinger N, Pei H and Rotter V (1997) The murine C''-terminally alternatively spliced form of p53 induces attenuated apoptosis in myeloid cells. Mol Cell Biol 17: 713-722

Bates S and Vousden KH (1996) p53 in signaling checkpoint arrest or apoptosis. Curr Opin Genet Dev 6: 12-18

Beck HP (1978) A new analytical method for determining duration of phases, rate of DNA synthesis and degree of synchronization from flow-cytometric data on synchronized cell populations. Cell Tissue Kinet 11: 139-148

Bosch FX, Ribes J and Borras J (1999) Epidemiology of primary liver cancer. Semin Liver Dis 19: 271-285

Buckbinder L, Talbott R, Velasco-Miguel S, Takenaka I, Faha B, Seizinger BR and Kley N (1995) Induction of the growth inhibitor IGF-binding protein 3 by p53. Nature 377: 646-649

國際替代計量

植物多醣萃取物對肝癌與T淋巴癌細胞增殖之影響

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