摘要
許多傳統中藥已知具有促進免疫的功效，本研究選擇八種在臨床使用之補益方劑，利用LPS誘導小鼠脾懸液細胞增生的試驗中，發現在12.5 μg/ml時，黃耆六一湯能增強88 %，具有最佳的促進免疫效果。 第一部分 ─ 進一步探討自然殺手細胞(Natural killer cells)毒殺活性試驗，也具有明顯增強效果，另對於體內白血球增生的試驗中，連續餵服28天之黃耆六一湯(400，800，1600 mg/kg)後，白血球亦明顯增生。此外再利用免疫抑制劑cyclophosphamide造成白血球減少症之動物模式中，黃耆六一湯尚具有延緩下降的趨勢及提早復原的能力。在保護肝細胞的實驗中，利用1.25 mM氧化劑t-BuOOH，造成正常肝細胞(Chang liver cells)的生長抑制，黃耆六一湯具有明顯保護作用。 第二部分 ─ 黃耆六一湯的組成為豆科的黃耆、甘草和鼠李科的大棗，其中甘草在促進免疫的效果，最為顯著，其主成分glycyrrhizin利用LPS誘導小鼠脾懸液細胞增生的試驗中，發現在0.39 μM時即對脾懸液細胞具有明顯的增生效果。所以我們選擇甘草的主成分glycyrrhizin及黃耆的成分formononetin，做為指標成份，利用高效率液相層析方法建立定量的分析的方法。而利用此方法檢測黃耆六一湯萃取物含glycyrrhizin 7.26±0.04 mg/g，formononetin 112.07±0.81 μg/g。此分析的方法可做為品質管制的參考依據。
並列摘要
Nowadays a lot of Chinese medicines have been proved to have the immuno-enhancement effects. In this study, eight tonic Chinese prescriptions, including Huang Qi Liu Yi Tang (HQLYT), were experimented by the method of MTT and the inducement of lipopolysaccharide (LPS). The results showed that HQLYT (12.5 μg/ml) had the best immuno-enhancement effect, which enhances 88% of the survival rate on spleen cells. In the assay of natural killer cell cytotoxicity, HQLYT obviously had its immuno-enhancement effect. Besides, in the assay of leucocyte measurement and the white blood cells (WBC) are significantly proliferated by HQLYT. Using cyclophosphamide to cause leucopenia, this research finds that HQLYT can delay the decline of WBC, and recover WBC back to normal stasis faster. This research also investigates and finds that HQLYT has the protective effect against t-BuOOH (1.25 mM) in Chang liver cells. HQLYT is composed of Astragali Radix (Leguminosae), Glycyrrhizae Radix, and Zizyphi Fructus (Rhamnaceae). Among these components, Glycyrrhizae Radix has the greatest immuno-enhancement effect. Therefore, glycyrrhizin, a main bioactive content of Glycyrrhizae Radix, is chosen to be a substance marker to detect the contents in HQLYT by high performance liquid chromatograph (HPLC). After HPLC-profile of HQLYT is analyzed, the component markers of glycyrrhizin and formononetin are found and the contents of them in HQLYT extract are 7.26±0.04 mg/g and 112.07±0.81 μg/g, respectively. By this HPLC system, it could be as a reference basis of quality control of HQLYT.
參考文獻
延伸閱讀
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