枸杞多醣體與藻藍素對阿斯匹靈誘導大鼠胃黏膜細胞RGM-1損傷之修復影響

Effects of Lycium babarum polysaccharides and C-phycocyanin on healing of aspirin-induced damage in rat gastric mucosal RGM-1 cells

王詩雅(Shih-Ya Wang)

指導教授 ： 趙振瑞

臺北醫學大學/營養學院/保健營養學研究所/碩士(2018年)

摘要

2011年台灣胃潰瘍盛行率為4.7%，而潰瘍的發生與黏膜障壁防禦功能降低有關，其中非類固醇抗發炎藥物的使用為造成黏膜障壁防禦功能降低的主因之一，其會增加活性氧屬生成，進而誘發胃損傷。而枸杞為一中藥材，其主要活性成份為枸杞多醣體(Lycium barbarum polysaccharides, LBP)。C型藻藍素(C-phycocyanin, C-Pc)則是由螺旋藻(Spirulina platensis)衍生的主要藻膽色素蛋白質，為具抗氧化能力的天然色素。故本研究欲探討預先添加不同濃度之LBP (100、300及800 μg/mL)或/與螺旋藻萃取物(30、250及750 μg/mL)培養24小時，對於aspirin所造成之大鼠胃黏膜RGM-1細胞損傷，是否能透過降低氧化傷害與抑制發炎反應之特性而達到修復胃黏膜損傷的效果。結果顯示，RGM-1細胞經25 mM aspirin誘發3小時後，NF-κB (p 65)與DNA之結合、iNOS活性、MDA、PGE2、mucin 1及EGF濃度相較於控制組皆顯著上升，SOD活性相較於控制組則顯著下降。然而，預先添加枸杞多醣(300 μg/mL)則可減輕因aspirin所誘發之細胞內iNOS活性上升，達到抗發炎之效果;而預先添加螺旋藻萃取物(30 μg/mL)除了亦能減輕因aspirin所誘發之細胞內iNOS活性上升，同時也可降低細胞內MDA之生成，達到抗發炎與降低氧化壓力之效果。也因此，此兩添加物進而預防非類固醇抗發炎藥物所造成之RGM-1細胞損傷，使得PGE2、MUC1及EGF等胃黏膜保護因子之表現較趨近於正常。

關鍵字

胃黏膜損傷 ； 阿斯匹靈 ； 枸杞多醣體 ； 藻藍素

並列摘要

Long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) is the major risk factor for the development of gastric ulcer. Lycium barbarum polysaccharides (LBP) have been reported to have antioxidation, immunomodulation, and regeneration actions. C-phycocyanin (C-Pc) is a major biliprotein pigment of Spirulina platensis. C-Pc has a variety of regulatory functions including antioxidation, anti-inflammation, and wound healing properties. The objectives of this study were to investigate the pre-treatment effects of LBP (100,300 and 800 μg/mL) and/or Spirulina extract (30, 250 and 750 μg/mL) for 24 hours on healing of aspirin-induced injury in rat gastric epithelial cell line RGM-1. The results showed that the incubation of 25 mM aspirin for 3 hours significantly increased the activities of NF-κB (p 65) DNA binding and iNOS, and concentrations of MDA, PGE2, mucin 1 and EGF. In addition, 25 mM aspirin significantly decreased SOD activity. The activity of iNOS was significantly inhibited by LBP (300 μg/mL) and Spirulina extract (30 μg/mL). Pretreatment with Spirulina extract (30 μg/mL) also significantly attenuated MDA production. Therefore, the pretreatment of LBP or C-Pc attenuates damage of RGM-1 cells induced by 25 mM aspirin, and returns concentrations of PGE2, MUC1 and EGF closer to normal status.

並列關鍵字

gastric mucosa damage ； aspirin ； Lycium barbarum polysaccharides ； C-phycocyanin

參考文獻

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