探討利用三碘甲腺胺酸誘導甲狀腺機能亢進鼠之 血液流變機轉

Hemorheological study of hyperthyroidism induced triiodothyronine in rats.

錢芝宇(Chih-Yu Chien)

指導教授 ： 劉得任

臺北醫學大學/口腔醫學院/生物醫學材料研究所/碩士(2008年)

摘要

甲狀腺機能亢進主要是因甲狀腺素分泌過量所造成，而過量的甲狀腺素會對身體造成影響，其中包含組織氧化傷害和異常的血液流變。 甲狀腺素會使粒線體發生氧化作用產生能量，進而產生大量的自由基，當紅血球受到自由基傷害時，除了會使紅血球攜氧能力降低，並且還會造成變形度下降，使血球不容易通過微血管，造成組織間的灌流量下降。血液流變異常主要因肝臟受到甲狀腺素影響，製造大量纖維蛋白原，造成血液中纖維蛋白原增加，而纖維蛋白原會使紅血球易聚集產生錢串，造成血液黏度上升，血流在血管中流速下降。本研究主要目的是在探討利用三碘甲腺胺酸誘導異常之血液流變之機轉。本試驗使用8周齡體重約300-330g之Sprague–Dawley公鼠(SD Rats)以口服途徑誘發甲狀腺機能亢進，SD鼠以口服途徑給予三碘甲腺胺酸(T3，10µg/100gBW) 連續20天後，在第21天和28天時，抽血測定血液生化值以及血液流變參數，使用Cone/Plate黏度儀檢測其血液黏度，以及利用雷射衍射法測量血球變形度和紅血球聚集度等相關參數，進而了解三碘甲腺胺酸誘導甲狀腺機能亢進鼠之血液流變參數異常之情形。目前本研究實驗結果發現老鼠給予口服三碘甲腺胺酸20天後，血清中三碘甲腺胺酸(T3)明顯上升(p<0.01)，但是甲狀腺素(L-thyroine)的含量卻明顯下降(p <0.01)。而纖維蛋白原(fibrinogen) 含量在三碘甲腺胺酸(T3)組有明顯增加的情形(p <0.01)，造成血液黏度上升。因甲狀腺機能亢進，也會引起氧化的傷害，故紅血球球膜有明顯的脂質過氧化之情形(p <0.01)以及全血中的自由基含量也相對上升(p <0.01)。

關鍵字

甲狀腺機能亢進 ； 血液流變學 ； 氧化傷害

並列摘要

A large amount of thyroine cost Hyperthyroidism, and it will affect the health of the human body; this includes oxidative damage and the increases of fibrinogen in blood. When oxidative damage affects red blood cell, except the function of carrying oxygen will decrease, also causes decrease in deformability. This affection will enables difficulty when blood cells pass through the capillary, and slowing down its flow rate in tissues. However, fibrinogen gathers the red blood cells decreases the blood flow rate, and increases the viscosity in blood. The goal of this research is to confer using triiodothyronine leads the unusual blood rheology. This research will be test on an 8 week old, 300-330g Sprague–Dawley (SD) Rats for 20 days by give it Triiodothyronine through its mouth. After giving the Triiodothyronine, we will draw blood from the rats and test its blood viscosity, deformability of the red blood cell, and gather rate of red blood cell. From the report we will learn the hyperfunction of Triiodothyronine in the rat’s blood. From the current testing result we found that 20 days after using Triiodothyronine on rats, Triiodothyronine obviously increased in its blood serum, but we also found the decrease in its L-thyroine. However, the fibrinogen in the rat’s blood could be raise and Blood viscosity increases in low shear rate. Hyperthyroidism cause lipid peroxidation in RBC membrane and free radical increases in blood.

並列關鍵字

hyperthyroidism ； hemorheology ； oxidation

參考文獻

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