兩種真菌醱酵液之生物活性成分研究

Chemical investigation of the fermented broths of Theissenia rogersii and Stilbohypoxylon elaeicola

張雅雯(Ya-Wen Chang)

指導教授 ： 李宗徽

臺北醫學大學/藥學院/生藥學研究所/碩士(2010年)

https://doi.org/10.6831/TMU.2010.00156

摘要

利用抑制一氧化氮合成酶的活性分析平台來篩選真菌培養株，藉以篩檢出具有抗發炎活性的真菌株，發現某些真菌株的醱酵培養液或菌絲體萃取物，對於RAW264.7細胞的產生一氧化氮具有顯著的抑制作用。遂選定：Theissenia rogersii（#92031201）及Stilbohypoxylon elaeicola（#173）兩株進行其活性成分研究。在選擇以麥芽抽取物（malt extract）為培養基加以擴大培養後，針對醱酵液所含代謝產物進行分析與分離，計獲得12個化合物，分別為： 2,5-dihydroxymethylfuran（1）、pycnidione（2）、maltol（3）、tyrosol（4）、hymatoxin K（5）、hymatoxin L（6）、elaeicolactone（7）、5-hydroxymethylfurfural（8）、elaeicolasdie A（9）elaeicolaside B（10）、elaeicolaside C（11）和 elaeicolaside D（12），其中屬於倍半萜之化合物7，及屬於二萜糖苷的化合物9、10及12，皆為新化合物。在100 μM的濃度下，10對於RAW264.7細胞的產生一氧化氮具有顯著的抑制活性且不具細胞毒性。此外，2及10對於A549肺腺癌細胞具有強的毒殺作用，其GI50分別為5.25 nM及9.79 μM。

關鍵字

真菌 ； 誘導型一氧化氮合成酶Theissenia rogersii ； Stilbohypoxylon elaeicola ； elaeicolaside ； elaeicolactone

並列摘要

The ethyl acetate extracts of the fermented broths of Theissenia rogersii (#92031201) and Stilbohypoxylon elaeicola (#173) were found to exhibit significant activity against inducible nitric oxide synthase (iNOS) in our preliminary screening. Therefore, bioassay-guided fraction and separation of the active component from these two broths were carried out which resulted in the isolation of twelve compounds. Their structures were elucidated to be 2,5-dihydroxymethylfuran (1), pycnidione (2), maltol (3), tyrosol (4), hymatoxin K (5), hymatoxin L (6), elaeicolactone (7), 5-hydroxymethylfurfural (8), elaeicolasdie A (9), elaeicolaside B (10), elaeicolaside C (11) and elaeicolaside D (12). Of these compounds identified, 7, 9, 10 and 12, classified as respective a sesquiterpene and three diterpene glycosides, were novel chemical entities. Compound 10 exhibited significant inhibitory activity on NO production of RAW264.7 cells without any cytotoxicity. In addition, 2 and 10 exerted potent cytotoxicity against an A549 lung cancer cell line with GI50 values of 5.25 nM and 9.79 μM, respectively.

並列關鍵字

isopimarane diterpene glycoside ； elaeicolaside ； elaeicolactone ； Theissenia rogersii ； Stilbohypoxylon elaeicola ； iNOS

參考文獻

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