間質幹細胞對大鼠脊髓損傷影響之評估

Assessment of the effect of mesenchymal stem cells on the functional recovery of rats with spinal cord injury

邱佩玲(Pei-Ling Chiu)

指導教授 ： 楊良友

臺北醫學大學/醫學院/醫學科學研究所/碩士(2011年)

https://doi.org/10.6831/TMU.2011.00094

摘要

嚴重的脊髓損傷常常導致半身不遂或是全身癱瘓，因此嚴重地降低病人以及照顧家人的生活品質。導致脊髓損傷的主要原因包括車禍、運動受傷、高處墜落或是槍擊所導致。在西方國家及台灣脊髓損傷的發生率每一年分別為每百萬人就有28~50位及22例新增案例。直到目前為止，甲基去氫氧化可體松（methylprednisolone, MP）仍是唯一經美國國家食品暨藥物檢驗局所核准用來治療急性脊髓損傷的藥物。然而近年來甲基去氫氧化可體松用來治療急性脊髓損傷的效果受到質疑，而且使用甲基去氫氧化可體松也造成不少的副作用，因此如何找到有效治療脊髓損傷的方法仍是當前醫學研究最重要的課題之一。近年來由於幹細胞的研究愈趨成熟，因此愈來愈多的研究者探討利用幹細胞來治療受損的神經組織。本研究論文的主要目的是探討在受傷後不同時間點給予人類基質幹細胞( mesenchymal stem cells, MSCs)是否可改善大鼠脊髓損傷的情形。本研究初步的結果顯示於脊髓損傷後馬上或四周後給予人類基質幹細胞明顯改善大鼠後肢的行動能力；並且給予人類基質幹細胞後能有效減少神經組織損傷之情形。我們的研究結果支持人類基質幹細胞將來很可能成為治療脊髓損傷有效方法的論述。

關鍵字

脊髓損傷

並列摘要

Spinal cord injuries (SCIs) are one of the most devastating medical conditions and severe SCI often results in permanent paraplegia or tetraplegia, which drastically decreases the quality of life of SCI patients and their caring family members. The major causes of SCI include motor vehicle accidents, sports-related activities, falls or gunshots. The annual incidence rate of SCI in western countries and in Taiwan are roughly 28 to 50 and 22 spinal cord injuries per million, respectively. Up to now, methylprednisolone (Cesare Campagnoli) is still the only FDA-approved drug for treating acute spinal cord injury. However, more and more neurosurgeons question the efficacy of MP on treating SCI patients. MP also causes severe side effects. Thus, how to promote the quality of life of SCI patients and how to improve the health conditions of SCI patients are two ultimate goals and challenges for SCI researchers. In this study, we evaluated if immediate or delayed (4 weeks after injury) implantation of human mesenchymal stem cells (MSCs) into the lesioned spinal cord improves the functional recovery of SCI rats. Our preliminary data showed that implantation of human MSCs into the lesioned spinal cord immediately or 4 weeks after injury significantly improved the functional recovery of SCI rats. Our data also showed that implantation of human MSCs into the lesion site significantly decreased the spinal lesion. Our results strongly suggest that implantation of human MSCs is a potential therapeutic treatment for SCI patients.

並列關鍵字

spinal cord injury

參考文獻

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